产品介绍 目录号 Serial | SCSP-5079 | 标识符 Identifier | CSTR:19375.09.3101HUMSCSP5079 | 描述 Description | 病患之前没有接受过医治。该细胞表达 HLA I 类和 II 类抗原。细胞早期传代时被牛支原体(莱氏无胆甾原体)污染,在冻存前使用牛莱氏无胆甾原体抗血清(A. laidlawiiantiserum)和卡那霉素治愈。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误,STR鉴定结果为:Amelogenin: X,X;CSF1PO: 10,11;D13S317: 13,13;D16S539: 12,12;D5S818: 12,12;D7S820: 10,11;THO1: 8,9.3;TPOX: 8,11;vWA: 16,17. | 动物种别 Organism | 人,白人 | 性别 Gender | 男性,68岁 | 组织来源 Tissue and Cell Type | 肺 | 形态 Morphology | 贴壁 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | 人肺癌细胞 DMS 114 完全培养液 配方(100 ml): Waymouth's MB 752/1 medium (Invitrogen, 11220-035)90 ml FBS (Gibco)10 ml 参考传代比例:1:3参考传代周期:3-5天参考换液频率:每3-4天换1次液冻存液配方:完全培养液95%,DMSO 5%气相:空气,95%;二氧化碳,5%。温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980.PubMed:6266631Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980.PubMed:6249655 |
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