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(S)-Thalidomide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
(S)-Thalidomide图片
CAS NO:841-67-8
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
(S)-(-)-Thalidomide
产品介绍
(S)-Thalidomide ((S)-(-)-Thalidomide) 是 Thalidomide 的 S-对映体。(S)-Thalidomide 具有免疫调节,抗炎,抗血管生成和促凋亡作用。(S)-Thalidomide 通过与 cereblon (CRBN) 结合诱导致畸作用。
生物活性

(S)-Thalidomide ((S)-(-)-Thalidomide) is the S-enantiomer of Thalidomide. (S)-Thalidomide has immunomodulatory, anti-inflammatory, antiangiogenic and pro-apoptotic effects[1][2][3]. (S)-Thalidomide induces teratogenic effects by binding tocereblon(CRBN)[4].

IC50& Target

Apoptosis[1]

体外研究
(In Vitro)

(S)-Thalidomide treatment results in a reduction in cell viability in U266 cells with an IC50of 362 μM[1].
(S)-Thalidomide treatment increased apoptosis in a dose-dependent manner in U266 cells[1].
There are changes in the expression profile of genes involved in angiogenesis and apoptosis, but the changes are most dramatic in the apoptotic genes. In particular, the expression of I-κB kinase is decreased by two-fold, which is associated with a four-fold decrease in NF-κB expression. (S)-Thalidomide increases the Bax:Bcl-2 ratio, also increases I-kB protein levels, and decreases NF-kB activity. A dramatic decrease in Bcl-2 expression with (S)-Thalidomide suggests a possible enhancement of cytotoxic effect if combined with other cytotoxic agents[1].

Cell Viability Assay[1]

Cell Line:U266 MM cells
Concentration:0 μM, 10 μM, 100 μM, 150 μM, 200 μM, 1000 μM
Incubation Time:3 days
Result:A reduction in cell viability was observed in U266 cells.

Apoptosis Analysis[1]

Cell Line:U266 MM cells
Concentration:100 μM, 150 μM, 200 μM, 1000 μM
Incubation Time:3 days
Result:Increased apoptosis in U266 cells.
体内研究
(In Vivo)

Thalidomide does cause limb reduction defects in chick embryos as long as the embryos are directly exposed to the drug. The most useful techniques are implanting Thalidomide-soaked beads into the embryo immediately adjacent to the limb territory or soaking presumptive chick limb territories in Thalidomide and then grafting the explants to a host embryo celom. Thalidomide affects the chick limb grafted to a host embryo in a dose response fashion. Furthermore, (S)-Thalidomide is more teratogenic than (R)-Thalidomide[1].

分子量

258.23

性状

Solid

Formula

C13H10N2O4

CAS 号

841-67-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 83.33 mg/mL(322.70 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.8725 mL19.3626 mL38.7252 mL
5 mM0.7745 mL3.8725 mL7.7450 mL
10 mM0.3873 mL1.9363 mL3.8725 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。