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8-Aminoadenosine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
8-Aminoadenosine图片
CAS NO:3868-33-5
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品名称
8-氨基腺苷
8-NH2-Ado
产品介绍
8-Aminoadenosine (8-NH2-Ado) 是一种 RNA 导向的核苷类似物,可降低细胞 ATP 水平并抑制mRNA合成。8-Aminoadenosine 阻断Akt/mTOR信号,并诱导 p53 非依赖性的自噬和细胞凋亡。8-Aminoadenosine 具有抗肿瘤活性。
生物活性

8-Aminoadenosine (8-NH2-Ado), a RNA-directed nucleoside analogue, reduces cellular ATP levels and inhibitsmRNA synthesis. 8-Aminoadenosine blocksAkt/mTORsignaling and inducesautophagyandapoptosisin a p53-independent manner. 8-Aminoadenosine has antitumor activity[1][2][3].

IC50& Target[1][2]

mTOR

 

体外研究
(In Vitro)

8-Aminoadenosine (8-NH2-Ado; 0.1-10 μM; for 48 h) has IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively[1].
8-Aminoadenosine (10 μM; for 24 h) induces significant apoptotic death of MCF-7 cells in p53-independent pathway. 8-Aminoadenosine causes PARP cleavage in MCF-7 cells[2].
8-Aminoadenosine (3 μM; 0.5-4 h) induces autophagy in the MM.1S cell line[1].
8-Aminoadenosine (3 μM; 2-16 h) causes a greater drop in ATP levels in the MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) causes a 50% reduction in glucose consumption in MM.1S cells[1].
8-Aminoadenosine (3 μM; 5 h) indicates a time-dependent decrease in GLUT1 expression at 5 h, whereas at 24 h there was a down-regulation of both transporters (GLUT1 and GLUT4) in MM.1S cells[1].
8-Aminoadenosine inhibits cell proliferation, activated cell death, and does not activate transcription of the p53 target gene p21 or increase protein levels of either p53 or p21[1].
The toxic effects of 8-Aminoadenosine require adenosine kinase activity to convert 8-Aminoadenosine to 8-NH2-ATP in adenosine kinase-deficient cells[1].

Cell Viability Assay[1]

Cell Line:MM.1S and U266 cells
Concentration:0.1, 0.3, 1, 3, 10 μM
Incubation Time:For 48 hours
Result:Had IC50s of 1.5 μM and 8.88 μM in MM.1S and U266 cells, respectively.

Apoptosis Analysis[2]

Cell Line:MCF-7 cells
Concentration:10 μM
Incubation Time:For 24 hours
Result:Induced significant apoptotic death.
Apoptosis was not inhibited by knockdown of functional p53.

Apoptosis Analysis[1]

Cell Line:MM.1S cell line
Concentration:3 μM
Incubation Time:0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 hours
Result:Induced the formation of LC3-II protein.
Caused the appearance of a population with a high AVO content with 1 μM for 24 hours.
分子量

284.27

Formula

C10H16N6O4

CAS 号

3868-33-5

中文名称

8-氨基腺苷;8-氨基腺苷酸

结构分类
  • Others
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
In Vitro: 

DMSO : 83.33 mg/mL(293.14 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.5178 mL17.5889 mL35.1778 mL
5 mM0.7036 mL3.5178 mL7.0356 mL
10 mM0.3518 mL1.7589 mL3.5178 mL