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astragalin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
astragalin图片
CAS NO:480-10-4
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议

产品名称
紫云英苷
Astragaline
3-Glucosylkaempferol
Kaempferol 3-β-D-glucopyranoside
产品介绍
Astragalin (Astragaline) 是一种具有抗炎、抗氧化、抗癌、抑菌活性的黄酮类化合物。Astragalin 抑制癌细胞增殖和迁移,诱导细胞凋亡 (apoptosis)。Astragalin 具有口服活性,具有保护神经和心脏,抵抗骨质疏松的作用。
生物活性

Astragalin (Astragaline) a flavonoid with anti-inflammatory, antioxidant, anticancer, bacteriostatic activity. Astragalin inhibitscancercells proliferation and migration, inducesapoptosis. Astragalin is orally active and provides nerve and heart protection, and resistance against and osteoporosis[1].

IC50& Target

p65

 

体外研究
(In Vitro)

Astragalin (0-80 μg/mL; 24 h, 48 h, 72 h) inhibits cancer cells viability and migration at 20, 40, 80 μM, and shows no cytotoxicity to normal human colon epithelial cell lines NCM460[1].
Astragalin (80 μg/mL; 4 h, 8 h) interrupts the NF-κB signaling pathway and inhibits NF-κB P65 transcriptional activity in HCT116 cells stimulated by TNF-α[1].
Astragalin (20, 40, 80 μg/mL; 48 h) induces HCT116 cells apoptosis and arrests cell cycle at G0/G1 phase[1].
Astragalin (20, 40, 80 μg/mL; 48 h) increases the level of apoptin in HCT116 and decreases the level of anti-apoptotic proteins in a dose-dependent manner[1].

Cell Viability Assay[1]

Cell Line:Human colon cancer cell lines (HCT116, LoVo, SW620, SW480, Caco2) and human colon epithelial cell lines NCM460
Concentration:0, 5, 10, 20, 40, 80 μg/mL
Incubation Time:24, 48, 72 hours
Result:Inhibits cancer cells (LoVo, SW620, SW480, Caco2, HCT116) activity at 20, 40, 80 μM.
Inhibited HCT116 cells with IC50s of 121.845, 87.908, 18.883 μg/mL at 24, 48, 72 hours, respectively.
Showed no cytotoxicity with NCM460 cells.

Cell Cycle Analysis[1]

Cell Line:Human colon cancer cell HCT116
Concentration:0, 20, 40, 80 μg/mL
Incubation Time:48 hours
Result:Arrested cell cycle at G0/G1.

Western Blot Analysis[1]

Cell Line:Human colon cancer cell HCT116
Concentration:80 μg/mL; pre-treated with 20 ng/mL TNF-α
Incubation Time:4 or 8 hours
Result:Increased the expression of pro-apoptotic proteins (caspase 3, caspase 6, caspase 7, caspase 8, caspase 9, P53, and Bax) and decreased the expression of anti-apoptotic proteins (cleaved caspase-3 and Bcl-2) in a dose-dependent manner.

Cell Migration Assay[1]

Cell Line:Human colon cancer cell HCT116
Concentration:0, 20, 40, 80 μg/mL
Incubation Time:24, 48, 72 hours
Result:Reduced release of MMP-2 and MMP-9 enzymes.
Inhibited cell migration with mobilities of 14.38, 13.44, and 5.29% with 20, 40, and 80 μg/mL concentrations, respectively.
体内研究
(In Vivo)

Astragalin (25, 50, and 75 mg/kg; p.o.; once every two days; 25 d) reduces the tumor volumes, tumor weight and tumor formation rates and decreases expression of p-NFκB and p-iκκα in nude mouse model with human colon cancer[1].

Animal Model:Male BALB/c nude mice (4-week-old) with HCT116 cells (s.c.)[1]
Dosage:0 mg/kg, 25 mg/kg, 50 mg/kg, 75 mg/kg
Administration:Oral gavage; once every two days; for 25 days
Result:Resulted tumor inhibition rates of 17.43%, 34.89% (p < 0.01), and 67.06% with 25 mg/kg, 50 mg/kg, 75 mg/kg dose administration.
Showed no effect on mouse weight.
分子量

448.38

性状

Solid

Formula

C21H20O11

CAS 号

480-10-4

中文名称

紫云英苷;紫云英甙;黄芪苷;山奈酚-3-葡萄糖苷

结构分类
  • Flavonoids
  • Flavonols
  • Phenols
  • Polyphenols
来源
  • Plants
  • Santalaceae
  • Thesium chinenseTurcz.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL(223.03 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.2303 mL11.1513 mL22.3025 mL
5 mM0.4461 mL2.2303 mL4.4605 mL
10 mM0.2230 mL1.1151 mL2.2303 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.58 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.58 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.58 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。