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ML334
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
ML334图片
CAS NO:1432500-66-7
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
LH601A
产品介绍
ML334 (LH601A) 是一种有效的,细胞可渗透的NRF2激活剂,通过抑制Keap1-NRF2蛋白质相互作用来发挥作用。ML334 以Kd值为 1μM 来结合 Keap1 Kelch 域。ML334 刺激NRF2表达和核易位并诱导抗氧化反应元件 (ARE) 活性。
生物活性

ML334 is a potent, cell permeable activator ofNRF2by inhibition ofKeap1-NRF2protein-protein interaction. ML334 binds to Keap1 Kelch domain with aKdof 1 μM. ML334 stimulatesNRF2expression and nuclear translocation and induces antioxidant response elements (ARE) activity[1][2].

IC50& Target

Ki: 1 μM (Keap1)[2]

体外研究
(In Vitro)

ML334 (LH601A; 50-100 μM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h[1].
ML334 (LH601A; 50-100 μM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells[1].
ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells[1].

RT-PCR[1]

Cell Line:HEK293 cells
Concentration:50 μM or 100 μM
Incubation Time:6 hours, 16 hours
Result:Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h.

Western Blot Analysis[1]

Cell Line:HEK293 cells
Concentration:50 μM or 100 μM
Incubation Time:16 hours
Result:Enhanced HO-1 and TRX1 protein expression at 16 h.
分子量

446.50

性状

Solid

Formula

C26H26N2O5

CAS 号

1432500-66-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 44.65 mg/mL(100.00 mM;Need ultrasonic)

Ethanol : 44.65 mg/mL(100.00 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.2396 mL11.1982 mL22.3964 mL
5 mM0.4479 mL2.2396 mL4.4793 mL
10 mM0.2240 mL1.1198 mL2.2396 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。