ML334 is a potent, cell permeable activator ofNRF2by inhibition ofKeap1-NRF2protein-protein interaction. ML334 binds to Keap1 Kelch domain with aK_dof 1 μM. ML334 stimulatesNRF2expression and nuclear translocation and induces antioxidant response elements (ARE) activity^[1][2].

Ki: 1 μM (Keap1)^[2]

ML334 (LH601A; 50-100 μM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h^[1].
ML334 (LH601A; 50-100 μM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells^[1].
ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells^[1].

446.50

Solid

C₂₆H₂₆N₂O₅

1432500-66-7

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

DMSO : 44.65 mg/mL(100.00 mM;Need ultrasonic)

Ethanol : 44.65 mg/mL(100.00 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。