L-DABA (L-2,4-Diaminobutyric acid) is a weekGABAtransaminase inhibitor with anIC₅₀of larger than 500 μM; exhibits antitumor activityin vivoandin vitro.

The tumor cells are irreversibly and totally damaged by incubation with 10 mM L-2,4-Diaminobutyric acid for 24 h at 37℃. The cell-destructive effect by L-DABA is probably due to an osmotic lysis induced by the non-saturated intracellular accumulation of L-DABA. The harmful effect of L-DABA could be abolished by concomitant incubation with L-alanine and L-methionine^[1]. Kinetic studies indicates that L-DABA is a non-linear, non-competitive inhibitor of GABA transaminase activity. The L-DABA-induced elevation of GABA levels parallels the inhibition of GABA transaminase activity^[2]. L-2,4-Diaminobutyric acid, an amino acid analogue, produceS a cytolytic effect with a human glioma cell line, SKMG-1, and normal human fibroblasts. The concentrations of L-DABA necessary to reduce the cell count to 50% of control following a 24-h incubation at 37℃ are 12.5 mM for the human fibroblasts and 20 mM for the glioma cell line^[3].

Treatment with L-DABA results in 43.4% reduction of tumor growth^[1]. L-DABA is a more effective inhibitor of GABA transaminasein vivothanin vitro^[2].

118.13

Solid

C₄H₁₀N₂O₂

1758-80-1

L-2,4-二氨基丁酸

• Ketones, Aldehydes, Acids

Room temperature in continental US; may vary elsewhere.

H₂O : 1 mg/mL(8.47 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (846.53 mM); Clear solution; Need ultrasonic