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Streptolysin-O
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Streptolysin-O图片
包装与价格：
包装价格(元)
20μg电议
100μg电议
1mg电议

产品介绍

Streptolysin-O Streptococcus Pyogenes Recombinant

PurityGreater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
SourceEscherichia Coli.
Phycical AppearanceSterile Filtered white lyophilized powder.
Shipping ConditionShipped at Room temp.
SynonymsStreptolysin O; Thiol-activated cytolysin; slo.
Amino Acid SequenceNKQNTASTET TTTNEQPKPE SSELTTEKAG QKTDDMLNSN DMIKLAPKEM PLESAEKEEK KSEDKKKSEE DHTEEINDKI YSLNYNELEV LAKNGETIEN FVPKEGVKKA DKFIVIERKK KNINTTPVDI SIIDSVTDRT YPAALQLANK GFTENKPDAV VTKRNPQKIH IDLPGMGDKA TVEVNDPTYA NVSTAIDNLV NQWHDNYSGG NTLPARTQYT ESMVYSKSQI EAALNVNSKI LDGTLGIDFK SISKGEKKVM IAAYKQIFYT VSANLPNNPA DVFDKSVTFK ELQRKGVSNE APPLFVSNVA YGRTVFVKLE TSSKSNDVEA AFSAALKGTD VKTNGKYSDI LENSSFTAVV LGGDAAEHNK VVTKDFDVIR NVIKDNATFS RKNPAYPISY TSVFLKNNKI AGVNNRTEYV ETTSTEYTSG KINLSHQGAY VAQYEILWDE INYDDKGKEV ITKRRWDNNW YSKTSPFSTV IPLGANSRNI RIMARECTGL AWEWWRKVID ERDVKLSKEI NVNISGSTLS PYGSITYK.
SolubilityIt is recommended to reconstitute the lyophilized Streptolysin-O in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
FormulationStreptolysin-O protein was lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Introduction

Streptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. Cholesterol may be needed for binding to host membranes, membrane insertion and pore formation. Streptolysin-O can be reversibly inactivated by oxidation.

Stability

Lyophilized Streptolysin-O although stable at room temperature for 3 weeks, should be stored desiccated below -18℃ . Upon reconstitution Streptolysin-O should be stored at 4℃ between 2-7 days and for future use below -18℃ . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

产品描述

Recombinant Streptococcus Pyogenes Streptolysin-O produced in E.coli is a single, non-glycosylated, polypeptide chain containing 538 amino acids and having a molecular mass of 60.1kDa.The Streptolysin-O is purified by proprietary chromatographic techniques.