硝基四唑蓝氯化物 (NBT) 是脱氢酶的底物;在蛋白质印迹和免疫组织染色过程中与碱性磷酸酶底物 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) 一起使用。
Cas No. | 298-83-9 |
别名 | 氯化硝基四氮唑兰,NBT; Nitro blue tetrazolium chloride |
化学名 | 3,3'-(3,3'-dimethoxy-[1,1'-biphenyl]-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium) |
Canonical SMILES | COC1=C([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=C([N+]([O-])=O)C=C4)C=CC(C5=CC(OC)=C([N+]6=NC(C7=CC=CC=C7)=NN6C8=CC=C([N+]([O-])=O)C=C8)C=C5)=C1 |
分子式 | C40H30N10O6o 2Cl |
分子量 | 817.63 |
溶解度 | ≥ 10mg/mL in Water with gentle warming, 70%DMF : ≥ 100 mg/mL (122.30 mM), DMSO : 33.33 mg/mL (40.76 mM) |
储存条件 | Store at -20°C,protect from light |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. |