STAT5-IN-2 是STAT5的抑制剂，在 K562 和 KU812 细胞中的EC50分别为 9 μM和 5 μM，在文献 1 的名称是 17f。STAT5-IN-2 具有高效的抗白血病活性。
| 生物活性 | STAT5-IN-2 is aSTAT5inhibitor, extracted from reference 1, example 17f. STAT5-IN-2 has potent antileukemic effect[1]. |
| IC50& Target[1] | STAT5 9 μM (EC50, in K562 cells) | STAT5 5 μM (EC50, in KU812 cells ) |
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体外研究
(In Vitro) | STAT5-IN-2 has EC50values of 9 μM and 5 μM in K562 and KU812 cells, respectively[1].
STAT5-IN-2 (100 nM-50 μM; 48 hours) has EC50values of 2.6 μM and 3.5 μM in KG1a and MV-4-11 cells, respectively[1].
STAT5-IN-2 (10 μM; 48 hours) induces apoptosis in all cell lines (K562, KU812, KG1a and MV-4-11 cells)[1].
STAT5-IN-2 (10 μM; 24 hours) blocks phosphorylation of STAT5[1].
Cell Viability Assay[1] | Cell Line: | KG1a and MV-4-11 cell lines | | Concentration: | 100 nM, 10 μM, 20 μM, 30 μM, 40 μM, 50 μM | | Incubation Time: | 48 hours | | Result: | Inhibited cell growth in KG1a and MV-4-11 cell lines |
Apoptosis Analysis[1] | Cell Line: | K562, KU812, KG1a and MV-4-11 cells | | Concentration: | 10 μM | | Incubation Time: | 48 hours | | Result: | Significantly increased the number of apoptotic cells. |
Western Blot Analysis[1] | Cell Line: | KG1a, MV-4-11 and KU812 cells | | Concentration: | 10 μM | | Incubation Time: | 24 hours | | Result: | Inhibited phosphorylation of STAT5 compared to no influence on phosphorylation level of STAT3, Akt and Erk1/2. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(251.57 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.5157 mL | 12.5783 mL | 25.1566 mL | | 5 mM | 0.5031 mL | 2.5157 mL | 5.0313 mL | | 10 mM | 0.2516 mL | 1.2578 mL | 2.5157 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (6.29 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (6.29 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
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