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SC-58125
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
SC-58125图片
CAS NO:162054-19-5
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
SC-58125 是一种有效的和选择性的环氧合酶2 (COX-2)的抑制剂,IC50值为 0.04 μM。SC-58125 在体外和体内均表现出抗肿瘤活性,还可以抑制炎症部位的水肿并具有缓解疼痛作用。
生物活性

SC-58125 is a potent and selective inhibitor ofcyclooxygenase 2 (COX-2), with anIC50of 0.04 μM. SC-58125 exhibits antitumor activity in vitro and in vivo. SC-58125 also can inhibit edema at the inflammatory site and has analgesic effect[1][2][3].

IC50& Target

hCOX-2

0.04 μM (IC50)

hCOX-1

>100 μM (IC50)

体外研究
(In Vitro)

SC-58125 (0.001-100 μM) has a high degree of selectivity for the inducible form of COX-2 (IC50=1 μM) over the COX-1 (IC50>100 μM)[1].
SC-58125 (10 μM; 20-140 s) is time-dependent and is complete by 1 min, with a half-maximal inhibition at 20 s[1].
SC-58125 (25-100 μM; 3 d) inhibits the in vitro growth of HCA-7 and LLC cells[3].
SC-58125 (100 μM; 12 h) induces G2arrest in LLC cells[3].
SC-58125 (25-100 μM; 3 d) decreases p34cdc2levels in HCA-7 cells[3].
SC-58125 (100 μM; 24 or 72 h) does not induce apoptosis of HCA-7 and LLC cells[3].

Cell Proliferation Assay[3]

Cell Line:HCA-7 and LLC cells
Concentration:0, 25, 50, 100 μM
Incubation Time:3 days
Result:Reduced the cell number and MTT activity in both cell lines in a dose-dependent manner.

Cell Cycle Analysis[3]

Cell Line:LLC cells
Concentration:100 μM
Incubation Time:12 hours
Result:Increased in the number of cells containing 4n DNA content in a dose- and time-dependent manner.
Reduced the number of mitotic figures.

Western Blot Analysis[3]

Cell Line:HCA-7 cells
Concentration:0, 25, 50, 100 μM
Incubation Time:3 days
Result:Resulted in a dose-dependent decrease in p34cdc2activity with strong inhibition, even at the lowest concentration.
体内研究
(In Vivo)

SC-58125 (10 mg/kg; i.p. every 48 h) inhibits the growth of established colorectal cancer xenografts in mice[3].
SC-58125 (10 mg/kg; a single i.p.) reduces tumor PGE2 levels in mice[3].
SC-58125 (10 mg/kg; a single i.p.) does not change the tumor levels of COX-1 and COX-2 protein in mice[3].

Animal Model:Athymic Sprague-Dawley mice are injected HCA-7 cells[3]
Dosage:10 mg/kg
Administration:I.p. every 48 h; at the time of tumor implantation or 2 and 4 weeks later
Result:Decreased the tumor growth rates significantly.
分子量

384.35

Formula

C17H12F4N2O2S

CAS 号

162054-19-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.