| 生物活性 | CalcitoninGene Related Peptide (CGRP) II, rat TFA, aCGRP receptoractivator, is a potent and long-lastingvasodilator.CalcitoninGene Related Peptide (CGRP) II TFA can be used in the research of cardiovascular diseases[1]. |
体外研究
(In Vitro) | Calcitonin Gene Related Peptide (CGRP) II, rat TFA induces vasodilator effects in small-diameter pig left anterior descending (LAD) coronary arteries with an EC50value of 0.56 nM[1].
Calcitonin Gene Related Peptide (CGRP) II, rat TFA relaxes the spontaneous tone in isolated internal anal sphincter (IAS) strips, with an EC50value of 83 μM[2].
Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rβCGRP) inhibits [125I]hαCGRP binding (IC50: 7 nM), and stimulates cAMP accumulation (EC50: 0.56 nM) in the cells co-expressing mRAMP1 (mouse receptor-activity-modifying-protein 1) and rCRLR (rat calcitonin receptor-like receptor)[3].
Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rat-βCGRP, 0.01-100 nM) induces endothelium-independent relaxations in the coronary arteries from male and female Sprague-Dawley rats, with an IC50value of 2.8 nM approximately[4].
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体内研究
(In Vivo) | Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rat-βCGRP, bolus injection, 0.3 μg/kg, at 10 min intervals) induces hypotension and vasodilatation in Male Sprague-Dawley rats[5].
| Animal Model: | Male Sprague-Dawley rats[5] | | Dosage: | 0.01 ng/kg-3 μg/kg (100 μL), succeeded by a 150 μL isotonic saline flush, at 10 min intervals. | | Administration: | A bolus injection | | Result: | Induced PA (pial artery) dilatation and increased in LCBFFlux(local cortical cerebral blood flow). |
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| 分子量 | |
| 性状 | |
| Formula | |
| Sequence | Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7) |
| Sequence Shortening | SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSKAF-NH2 (Disulfide bridge: Cys2-Cys7) |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Sealed storage, away from moisture | Powder | -80°C | 2 years | | -20°C | 1 year |
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Solvent & Solubility | In Vitro: H2O Peptide Solubility and Storage Guidelines: 1. Calculate the length of the peptide. 2. Calculate the overall charge of the entire peptide according to the following table: | | Contents | Assign value | | Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 | | Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 | | Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution: | Overall charge of peptide | Details | | Negative (<0) | 1. Try to dissolve the peptide in water first.
2. If water fails, add NH4OH (<50 μL).
3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. | | Positive (>0) | 1. Try to dissolve the peptide in water first.
2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. | | Zero (=0) | 1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
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