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Calcitonin Gene Related Peptide(CGRP)II,rat TFA
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
包装与价格:
包装价格(元)
5mg电议
10mg电议

产品介绍
Calcitonin Gene Related Peptide (CGRP) II, rat TFA,一种 CGRP 受体 (CGRP receptors) 激活剂,是一种有效且持久的血管扩张剂。Calcitonin Gene Related Peptide (CGRP) II, rat TFA 可用于心血管疾病的研究。
生物活性

CalcitoninGene Related Peptide (CGRP) II, rat TFA, aCGRP receptoractivator, is a potent and long-lastingvasodilator.CalcitoninGene Related Peptide (CGRP) II TFA can be used in the research of cardiovascular diseases[1].

体外研究
(In Vitro)

Calcitonin Gene Related Peptide (CGRP) II, rat TFA induces vasodilator effects in small-diameter pig left anterior descending (LAD) coronary arteries with an EC50value of 0.56 nM[1].
Calcitonin Gene Related Peptide (CGRP) II, rat TFA relaxes the spontaneous tone in isolated internal anal sphincter (IAS) strips, with an EC50value of 83 μM[2].
Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rβCGRP) inhibits [125I]hαCGRP binding (IC50: 7 nM), and stimulates cAMP accumulation (EC50: 0.56 nM) in the cells co-expressing mRAMP1 (mouse receptor-activity-modifying-protein 1) and rCRLR (rat calcitonin receptor-like receptor)[3].
Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rat-βCGRP, 0.01-100 nM) induces endothelium-independent relaxations in the coronary arteries from male and female Sprague-Dawley rats, with an IC50value of 2.8 nM approximately[4].

体内研究
(In Vivo)

Calcitonin Gene Related Peptide (CGRP) II, rat TFA (rat-βCGRP, bolus injection, 0.3 μg/kg, at 10 min intervals) induces hypotension and vasodilatation in Male Sprague-Dawley rats[5].

Animal Model:Male Sprague-Dawley rats[5]
Dosage:0.01 ng/kg-3 μg/kg (100 μL), succeeded by a 150 μL isotonic saline flush, at 10 min intervals.
Administration:A bolus injection
Result:Induced PA (pial artery) dilatation and increased in LCBFFlux(local cortical cerebral blood flow).
分子量

3919.33

性状

Solid

Formula

C165H268F3N51O52S2

Sequence

Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7)

Sequence Shortening

SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSKAF-NH2 (Disulfide bridge: Cys2-Cys7)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.