Phentolamine mesylate (Phentolamine methanesulfonate) is a reversible, non-selective, and orally active blocker ofα1andα2adrenergic receptorthat expands blood vessels to reduce peripheral vascular resistance. Phentolamine mesylate can be used for the research of pheochromocytoma-related hypertension, heart failure and erectile dysfunction^[1][2][3].

α-adrenergic^[1]

Phentolamine (0.1-1 μM) inhibits the response to Clonidine in rat ileum, with the pA₂and pK_Bof 7.92 and 8.07, respectively^[3].
Phentolamine (10-70 μg/mL; 48 h) inhibits proliferation of HDMECs and HBMECs in a dose-dependent manner, with IC₅₀s of 50 μg/mL and 30 μg/mL without toxicity, respectively^[4].
Phentolamine (10-20 or 20-40 μg/mL; 12-48 h) significantly delays scratch wound closure of HBMECs and HDMECs in a dose-dependent manner^[4].
Phentolamine (30 or 50 μg/mL; 4-12 h) inhibits tube formation of HBMECs and HDMECs^[4].

Phentolamine (1 mg/kg; i.v.) produces hypotension and tachycardia in rats^[4].

377.46

Solid

C₁₈H₂₃N₃O₄S

65-28-1

甲磺酸酚妥拉明

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

H₂O : ≥ 50 mg/mL(132.46 mM)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (264.93 mM); Clear solution; Need ultrasonic
• 2.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (6.62 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。