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Spadin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:1270083-24-3
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
Spadin 是一种从血液中释放的前肽衍生的天然肽,是一种有效的TREK-1 通道阻滞剂,其IC50值为 10 nM。Spadin 增强小鼠中缝背核 5-HT 神经传递,诱导海马 CREB 激活和神经发生。Spadin 可用于抗抑郁研究。
生物活性

Spadin, a natural peptide derived from a propeptide released in blood, is a potentTREK-1 channelblocker withIC50value of 10 nM. Spadin enhances dorsal raphe nucleus 5-HT neurotransmission in mice and induces hippocampal CREB activation and neurogenesis. Spadin can be used for antidepressant research[1][2].

体外研究
(In Vitro)

Spadin (100 nM; COS-7 cells) has inhibitory effect of spadin on the TREK-1 channel and blocks 63% of the TREK-1 current stimulated by arachidonic acid[1].
Spadin (100 nM) blocks the TREK-1 channels activity in CA3 hippocampal neurons on brain slices of wild-type mice[1].

体内研究
(In Vivo)

Spadin (10 μM; i.p.; for 30 min; male C57Bl/6J and TREK-1 deficient mice) increases of the 5-HT neuron firing rate in the dorsal raphe nucleus (DRN)[2].
Spadin (0.01-100 μM; ICV, i.p. and i.v.; daily, for 7 days; male C57Bl/6J and TREK-1 deficient mice) has anti-depressant behavior in mice[2].

Animal Model:Male C57Bl/6J and TREK-1 deficient mice[2]
Dosage:10 μM
Administration:Intraperitoneal injection; for 30 min
Result:Increased of the 5-HT neuron firing rate in the dorsal raphe nucleus (DRN).
Animal Model:Male C57Bl/6J and TREK-1 deficient mice[2]
Dosage:0.01-100 μM
Administration:Intracerebroventrical injection, intraperitoneal injection and intravenous injection; daily, for 7 days
Result:Had any effect on mouse locomotion analyzed in short- or long-time after the drug injection.
分子量

2012.34

Formula

C96H142N26O22

CAS 号

1270083-24-3

Sequence Shortening

YAPLPRWSGPIGVSWGLR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

 ContentsAssign value
Acidic amino acidAsp (D), Glu (E), and the C-terminal -COOH.-1
Basic amino acidArg (R), Lys (K), His (H), and the N-terminal -NH2+1
Neutral amino acidGly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)0

3.  Recommended solution:

Overall charge of peptideDetails
Negative (<0)1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.