CHIC35, an analog of EX-527, is a potent and selective inhibitor ofSIRT1(IC₅₀=0.124 μM). CHIC35 shows potential selective inhibition againstSIRT1overSIRT2(IC₅₀=2.8 μM) orSIRT3(IC₅₀>100 μM)^[1]. CHIC35 has anti-inflammatory effects and can be used for CHARGE syndrome research^[1][2].

CHIC-35 (0.5 μM; 16 hours) increases acetylation of histone H4 in BMDMs similar to Cambinol (200 μM)^[1].
CHIC-35 (5 μM; 72 hours) exhibits no significant difference in the survival of embryos at early stages^[2].
Zebrafish embryos are microinjected with 2.4 ng of chd7 MO to develop to different stages of development.chd7 morphant embryos are treated with CHIC-35 from 8hpf to 24hpf. CHIC-35 (5 μM) is removed at 24hpf and embryos are incubated in fresh egg water until 4dpf. The chd7 morphant larvae has a severely reduced and disrupted pattern of cartilage elements in comparison to the control, CHIC-35 shows partial recovery in craniofacial cartilage elements^[2].
At 4dpf, zebrafish embryos show a well-formed lower jaw in controls, while chd7morphants exhibits reduced lower jaw. Treatment with CHIC-35 (5 μM) rescues the expression of sox9a inchd7morphants^[2].
Nearly 30% of chd7 morphant embryos (24hpf to 72hpf) shows a near complete loss of isl2a expression in the cranial region compared to 10% of the wildtype controls. CHIC-35 reduces this to 7.5% significantly. However, CHIC-35 shows no discernible effect on the enteric neurons marked by Tg^[2].

262.73

C₁₄H₁₅ClN₂O

848193-72-6

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