Prostratin 是一种天然的二萜类物质，为PKC的激活剂，Ki值为 12.5 nM，同时可抑制HIV-1的活性。
| 生物活性 | Prostratin, a natural terpenoid compound, is aPKCactivator, with aKiof 12.5 nM and shows inhibitory effect onHIV-1. |
| IC50& TargetPKC
PKC[1] | |
体外研究
(In Vitro) | Prostratin 抑制 [3H]PDBu 与 CEM 细胞的结合，Ki为 210 nM[1]。
Prostratin (125-1000 nM) 剂量依赖性地抑制急性髓性白血病 (AML) 细胞系 (HL-60、NB4 和 U937 细胞) 的生长。Prostratin (125-100 nM) 诱导 AML 细胞的 G1 期阻滞并影响 HL-60 细胞中的细胞周期相关分子 (pRb 磷酸化、CDK 和 p21)。Prostratin 还通过激活 PKC 导致 AML 细胞系分化。此外，MEK/ERK/MAP 信号通路的 PKC 依赖性激活需要 Prostratin 诱导的分化[2]。
Prostratin 诱导需要 PKD3 活性形式的 HIV-1 转录激活。Prostratin 还通过新型 PKC 亚家族的 PKCε 激活 PKD3[3]。
Cell Viability Assay[2] | Cell Line: | HL-60, NB4 and U937 cells | | Concentration: | 125 nM, 250 nM, 500 nM, 1000 nM | | Incubation Time: | 24 hours, 48 hours, 72 hours | | Result: | Dose-dependently inhibited the growth of acute myeloid leukemia (AML) cell lines. |
Cell Cycle Analysis[2] | Cell Line: | HL-60, NB4 and U937 cells | | Concentration: | 125 nM, 250 nM, 500 nM, 1000 nM | | Incubation Time: | 24 hours | | Result: | Induced a G0/G1 phase accumulation in a concentration-dependent manner. |
Western Blot Analysis[2] | Cell Line: | HL-60 cells | | Concentration: | 125 nM, 250 nM, 500 nM, 1000 nM | | Incubation Time: | 24 hours | | Result: | Affected the cell-cycle-related molecules (pRb phosphorylation, CDKs, and p21) in HL-60 cells. |
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| 来源 | - Plants
- Thymelaeaceae
- Wikstroemia chamaedaphneMeisn.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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