ML-9 Free Base

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ML-9 Free Base

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

110448-31-2

包装与价格：

包装	价格(元)
100mg	电议
250mg	电议
500mg	电议

产品介绍

ML-9 (Free Base) 是 Akt 激酶的选择性强效抑制剂，可抑制肌球蛋白轻链激酶 (MLCK) 和基质相互作用分子 1 (STIM1) 的活性。 ML-9 (Free Base) 抑制 MLCK，PKA 和 PKC 活性，K_i值分别为 4、32 和 54 μM。 ML-9 通过刺激自噬小体的形成并抑制其降解来诱导自噬 (autophagy)。

生物活性

ML-9 (Free Base) is a selective and potent inhibitor ofAktkinase, inhibitsmyosinlight-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity^[3]. ML-9 (Free Base) inhibits inhibits MLCK,PKAandPKCactivity withK_ivalues of 4, 32 and 54 μM, respectively^[1]. ML-9 (Free Base) inducesautophagyby stimulating autophagosome formation and inhibiting their degradation^[3].

体外研究
(In Vitro)

ML9 (Free Base) (0-100 μM; 0-24 hours) has no reduction in cardiomyocyte viability, 50-100 μM significantly induces cell death^[2].
ML9 (Free Base) (50 μM; 1-4 hours) significantly increases cleaved caspase-3 levels, decreased STIM1 protein levels by about 42%^[2].

Cell Viability Assay^[1]

Cell Line:	Neonatal rat ventricular myocytes (NRVM) cells
Concentration:	0, 10, 50 and 100 μM
Incubation Time:	0, 1, 4, 8 and 24 hours
Result:	Decreased cell viability at 50–100 μM concentration.

Apoptosis Analysis^[1]

Cell Line:	Neonatal rat ventricular myocytes (NRVM) cells
Concentration:	50 μM
Incubation Time:	0, 1, 4, 8 hours
Result:	Induced cardiomyocyte death through necrosis and apoptosis.

分子量

324.83

Formula

C₁₅H₁₇ClN₂O₂S

CAS 号

110448-31-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.