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SAHA-OH
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
SAHA-OH图片
包装与价格:
包装价格(元)
100mg电议
250mg电议
500mg电议

产品介绍
SAHA-OH 是一种选择性的HDAC6抑制剂 (IC50=23 nM),与 HDAC1、2、3 和 8 相比,其对 HDAC6 有 10-47 倍的选择性。SAHA-OH 具有抗炎活性,并可减少巨噬细胞的凋亡。
生物活性

SAHA-OH is a selectiveHDAC6inhibitor (IC50=23 nM), shows a 10- to 47-fold selectivity forHDAC6compared toHDAC1, 2, 3, and 8. SAHA-OH shows anti-inflammatory activity, and attenuates macrophageapoptosis[1].

IC50& Target[1]

HDAC6

23 nM (IC50)

HDAC1

237 nM (IC50)

HDAC3

359 nM (IC50)

HDAC2

399 nM (IC50)

HDAC8

1070 nM (IC50)

体外研究
(In Vitro)

SAHA-OH (0.67-10.76 μM; 51 h) shows inhibition in bone marrow macrophages[1].
SAHA-OH (0.01 μM; 3 h) treatment in BMMOs (bone marrow macrophages) reduces IL-6, TNFα, IFNβ, IL-1β, IL-10, MCP-1 (CCL2) and GROα (CXCL1) secretions[1].
SAHA-OH (10 μM; 4 or 9 h) treatment induces acetylation of cytoplasmic α-tubulin and nuclear histone H3[1].
SAHA-OH (0-30 μM; 3 h) treatment attenuates macrophage apoptosis[1].
SAHA-OH (0-30 μM; 3 h) treatment attenuates B cell death[1].

Cell Viability Assay[1]

Cell Line:BMMOs (bone marrow macrophages)
Concentration:0.67-10.76 μM
Incubation Time:51 h
Result:Showed IC50value in unstimulated BMMOs of 1.26 μM, and showed IC50value in LPS-stimulated BMMOs of 10.76 μM.

Apoptosis Analysis[1]

Cell Line:BMMOs (bone marrow macrophages)
Concentration:0-30 μM
Incubation Time:3 h
Result:Resulted in a 24- to 26-fold increase in cellular viability as compared to the SAHA treatment.

Cell Cytotoxicity Assay[1]

Cell Line:B cells
Concentration:0-30 μM
Incubation Time:3 h
Result:Resulted in a 5-fold enhancement in viability and a 3-fold decrease in cell death for the B cell population.

Western Blot Analysis[1]

Cell Line:BMMOs (bone marrow macrophages)
Concentration:10 μM
Incubation Time:4 or 9 h
Result:Resulted in the acetylation of α-tubulin.
Induced the acetylation of histone H3 compared to no treatment (NT).
体内研究
(In Vivo)

SAHA-OH (intraperitoneal injection; 50 mg/kg; once) treatment prevents splenic organ damage, and reduces plasma proinflammatory cytokine levels in LPS-induced endotoxemia mouse model[1].

Animal Model:LPS-induced endotoxemia mouse model[1]
Dosage:50 mg/kg
Administration:Intraperitoneal injection; 50 mg/kg; once
Result:Reduced proinflammatory cytokine secretions by about 50% compared to no treatment (NT) control mice.
Showed similar architecture as no treatment (NT) control and displayed well-organized lymphoid follicles.
分子量

294.35

Formula

C15H22N2O4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.