Top/HDAC-IN-2 (45b) 是一种Top和HDAC的双重抑制剂，具有强大的抗肿瘤活性并诱导细胞凋亡。
| 生物活性 | Top/HDAC-IN-2 (45b), aTop andHDACdual inhibitor, exhibits potentantitumoractivities and inducesapoptosis[1]. |
| IC50& Target | HDAC1 0.004 μM (IC50) | HDAC2 0.005 μM (IC50) | HDAC3 1.10 μM (IC50) | HDAC6 0.15 μM (IC50) | HDAC8 4.5 μM (IC50) | Top1 | Topoisomerase II |
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体外研究
(In Vitro) | Top/HDAC-IN-2 (45b) shows antitumor activities against the HCT116 (IC50= 0.23 μM), MCF-7 and A549 cells[1].
Top/HDAC-IN-2 (45b) (0-5 μM, 24h) arrests the HCT116 cell cycle at the G2 phase in a concentration-dependent manner[1].
Top/HDAC-IN-2 (45b) (0-2.5 μM, 24h) induces the apoptosis in HCT116 cell line in a concentration-dependent manner[1].
Top/HDAC-IN-2 (45b) (0-5 μM, 24h) inhibits HDAC in living cells[1].
Cell Cycle Analysis[1] | Cell Line: | HCT116 | | Concentration: | 0.1, 2.5 and 5 μM | | Incubation Time: | 24 h | | Result: | Arrested the HCT116 cell cycle at the G2 phase in a concentration-dependent manner. |
Apoptosis Analysis[1] | Cell Line: | HCT116 | | Concentration: | 0.1, 0.5 and 2.5 μM | | Incubation Time: | 24 h | | Result: | Effectively induced the apoptosis in HCT116 cell line in a concentration-dependent manner. |
Western Blot Analysis[1] | Cell Line: | HCT116 | | Concentration: | 1 μM and 5 μM | | Incubation Time: | 24 h | | Result: | Concentration-dependently increased the level of acetyl-H3 and acetyl-H4 in HCT116 cells. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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