AES-135 是一种基于羟肟酸的泛HDAC抑制剂，可延长胰腺癌原位小鼠模型的存活时间。AES-135 抑制 HDAC3、HDAC6、HDAC8 和 HDAC11，IC50范围为190-1100 nM。
| 生物活性 | AES-135, a hydroxamic acid-based pan-HDACinhibitor, prolongs survival in an orthotopic mouse model of pancreaticcancer. AES-135 inhibitsHDAC3,HDAC6,HDAC8, andHDAC11withIC50s ranging from 190-1100 nM[1]. |
| IC50& Target[1] | HDAC6 190 nM (IC50) | HDAC11 636 nM (IC50) | HDAC3 654 nM (IC50) | HDAC8 1100 nM (IC50) |
|
体外研究
(In Vitro) | AES-135 inhibits cancer cells growth with IC50values of 2.3 μM, 1.4 μM, 0.27 μM, 0.94 μM, 1.9 μM, 2.72 μM, 2.1 μM, 15.0 μM, 1.6 μM and 19.2 μM for BT143, BT189, D425, D458, MV4-11, MOLM-13, MDA-MB-231, K562, PC-3 and MRC-9 cells, respectively[1].
|
体内研究
(In Vivo) | AES-135 (50 mg/kg; intraperitoneal injection; 5 days a week; for 1 month) treatment significantly increases survival of C57Bl/6 mice implanted with KPC2 cells[1].
NSG mice are dosed with a single 20 mg/kg intraperitoneal (IP) injection, and blood is taken at 0.5 h, 1 h, 2 h, 4 h, 8 h, and 24 h. AES-135 achieved μM concentrations in the blood, reaching Cmax7452 ng/mL (10.74 μM) within 30 min, which is sustained for 8 h. The blood concentration of AES-135 is dose dependent, achieving an average of 323 ng/mL (0.47 μM) with 10 mg/kg dosing and 1829 ng/mL (2.64 μM) with 40 mg/kg. AES-135 shows an impressive pharmacokinetic profile in mice with an in vivo half-life of 5.0 h[1].
| Animal Model: | C57Bl/6 mice injected with KPC2 cells[1] | | Dosage: | 50 mg/kg | | Administration: | Intraperitoneal injection; 5 days a week; for 1 month | | Result: | Significantly increased survival of mice. |
|
| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
|
| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(144.16 mM;Need ultrasonic) 配制储备液 | 1 mM | 1.4416 mL | 7.2081 mL | 14.4163 mL | | 5 mM | 0.2883 mL | 1.4416 mL | 2.8833 mL | | 10 mM | 0.1442 mL | 0.7208 mL | 1.4416 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (3.60 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (3.60 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
m.cnreagent.com