Hematein 是苏木精的氧化产物，可作为染料。Hematein 是一种变构酪蛋白激酶 II抑制剂，IC50为 0.74 μM。Hematein 抑制Akt/PKB Ser129磷酸化，Wnt/TCF途径并增加肺癌细胞的凋亡。
| 生物活性 | Hematein is a oxidation product of hematoxylin acted as a dye[1]. Hematein is an allostericcasein kinaseIIinhibitor with anIC50of 0.74 μM. Hematein inhibitsAkt/PKB Ser129phosphorylation, theWnt/TCFpathway and increasesapoptosisin lungcancercells[2]. |
| IC50& Target[2] | |
体外研究
(In Vitro) | Hematein (10-100 μM; 14 days) inhibits cells growth in A427 lung cancer cells[1].
Hematein (50 and 100 μM; 48 hours) induces apoptosis in A427 lung cancer cells[1].
Hematein (50 and 100 μM; 48 hours) inhibits CK2-specific Akt phosphorylation[1].
Cell Viability Assay[1] | Cell Line: | A427 (HTB-53) cell line | | Concentration: | 10-100 μM | | Incubation Time: | 14 days | | Result: | Inhibited cells growth. |
Apoptosis Analysis[1] | Cell Line: | A427 (HTB-53) cell line | | Concentration: | 50 and 100 μM | | Incubation Time: | 48 hours | | Result: | Induced apoptosis in A427 lung cancer cells. |
Western Blot Analysis[1] | Cell Line: | A427 (HTB-53) cell line | | Concentration: | 50 and 100 μM | | Incubation Time: | 48 hours | | Result: | Inhibited CK2-specific Akt phosphorylation. |
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体内研究
(In Vivo) | Hematein (intraperitoneal injections; 50 mg/kg; twice a week for 6 weeks) inhibits tumor growth in A427 lung cancer cell xenografts[1].
| Animal Model: | Female athymic BALB/c nude mice (6-week-old)[1] | | Dosage: | 50 mg/kg | | Administration: | Intraperitoneal injections; twice a week for 6 weeks | | Result: | Inhibited tumor growth in A427 lung cancer cell xenografts. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(333.04 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.3304 mL | 16.6522 mL | 33.3045 mL | | 5 mM | 0.6661 mL | 3.3304 mL | 6.6609 mL | | 10 mM | 0.3330 mL | 1.6652 mL | 3.3304 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.33 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 *以上所有助溶剂都可在本网站选购。
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