Aurora A inhibitor 2 (Compound 16h) 是一个有效的Aurora A kinase抑制剂，其IC50值为 21.94 nM。Aurora A inhibitor 2 诱导 MDA-MB-231 细胞 caspase 依赖性凋亡 (apoptosis)。
| 生物活性 | Aurora A inhibitor2 (Compound 16h) is a potentAurora Akinaseinhibitor with anIC50of 21.94 nM.Aurora A inhibitor2 induces caspase-dependentapoptosisin MDA-MB-231 cells[1]. |
| IC50& Target | Aurora A 21.94 nM (IC50) | Aurora B 273.18 nM (IC50) |
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体外研究
(In Vitro) | Aurora A inhibitor 2 (Compound 16h) (0-20 μM, 48 h) shows potent antiproliferative activity against various human cancer cells, and inhibits colony formation[1].
Aurora A inhibitor 2 (0-4 μM, 24 h) inhibits the expression of phosphorylation of Aurora A and Histone H3 in a dose-dependent manner, and induces G2/M cell cycle arrest[1].
Aurora A inhibitor 2 (0-4 μM, 48 h) induces obvious apoptosis in MDA-MB-231 cells in a concentration-dependent manner[1].
Cell Cytotoxicity Assay[1] | Cell Line: | Breast cancer MDA-MB231, prostate cancer PC3, and neuroblastoma SH-SY5Y cells | | Concentration: | 0-20 μM | | Incubation Time: | 48 h | | Result: | Displayed potent antiproliferative activity with IC50values of 0.38 ± 0.08, 1.09 ± 0.24, and 0.77 ± 0.12 μM against MDA-MB-231, PC3, and SH-SY5Y cells. |
Western Blot Analysis[1] | Cell Line: | MDAMB-231 | | Concentration: | 1, 2, and 4 μM | | Incubation Time: | 24 h | | Result: | A dose-dependent and significant reduction in the phosphorylation of Aurora A and Histone H3 was observed. Significantly increased the levels of cleaved caspase 3/9 and cleaved-PARP. |
Cell Cycle Analysis[1] | Cell Line: | MDAMB-231 | | Concentration: | 1, 2, and 4 μM | | Incubation Time: | 24 h | | Result: | Dose-dependently increased the and population of cells in the G2/M phase. |
Apoptosis Analysis[1] | Cell Line: | MDAMB-231 | | Concentration: | 1, 2, and 4 μM | | Incubation Time: | 48 h | | Result: | Induced obvious apoptosis in a concentration-dependent manner. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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