MPM-1 是海洋生物Eusynstyelamides模拟物，是一种有效的抗癌剂。MPM-1 可通过诱导坏死样死亡，在体外快速杀死癌细胞。MPM-1具有诱导免疫原性细胞死亡的能力。MPM-1在癌细胞中干扰细胞自噬 (autophagy) 和溶酶体膨胀。
| 生物活性 | MPM-1, a marineEusynstyelamidesmimic, is a potent anticancer agent. MPM-1 can rapidly killcancercellsin vitroby inducing a necrosis-like death. MPM-1 has the ability to induce immunogenic cell death. MPM-1 causes perturbation ofautophagyand lysosomal swelling incancercells[1]. |
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体外研究
(In Vitro) | MPM-1 (0-50 μM; 4 h) has cytotoxicity against various human cancer cell lines[1].
MPM-1 (8.5 and 17.0 μM; 1-6 h) causes perturbation of autophagy and lysosomal swelling[1].
MPM-1 induces the release and exposure of damage-associated molecular patterns (DAMPs) related to immunogenic cell death[1].
Cell Cytotoxicity Assay[1] | Cell Line: | Jurkat, Ramos, HSC-3, MCF-7, A375, et al. | | Concentration: | 0-50 μM | | Incubation Time: | 4 h | | Result: | Exhibited potent cytotoxicity against Jurkat, Ramos, HSC-3, MCF-7, A375 with IC50s of 6.62 ± 1.60 μM, 7.53 ± 2.01 μM, 8.53 ± 0.57 μM, 14.06 ± 2.71 μM, 14.52 ± 0.22 μM, respectively. |
Immunofluorescence[1] | Cell Line: | HSC-3 | | Concentration: | 8.5 and 17.0 μM | | Incubation Time: | 1, 2, 4 and 6 h | | Result: | Significantly increased the total number of autophagy markers p62 (substrate of autophagy) and LC3B.
Caused the distribution of the lysotracker dye more diffuse and less intense, indicating that lysosomal morphology was influenced. |
Western Blot Analysis[1] | Cell Line: | Ramos and HSC-3 | | Concentration: | 8.5 μM for Romas and 17.0 μM for HSC-3 | | Incubation Time: | 0.5, 1, 2, 3 and 4 h | | Result: | Increased the release of high mobility group box 1 (HMGB1) from cells. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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