P53R3 is a potentp53 reactivatorand restores sequence-specific DNA binding of p53 hot spot mutants, including p53^R175H, p53^R248W and p53^R273H. P53R3 induces p53-dependent antiproliferative effects with much higher specificity than PRIMA-1. P53R3 enhances the recruitment of wild-type p53 and p53^M237I to several target gene promoters. P53R3 strongly enhances the mRNA, total protein and cell surface expression of thedeath receptordeath receptor 5(DR5). P53R3 is used forcancerresearch^[1].

P53R3 (10 μg/ml; 24 hours; in the absence or presence of the unlabelled p53 consensus oligonucleotide) restores p53-specific DNA binding activity to p53^R273H(a DNA contact mutant) and p53R175H (a structural mutant) in WiDr colon tumour cells harbouring p53^R273H and KLE cells with p53R175H^[1].
P53R3 (1-33 μg/ml; 24 hours) inhibits the proliferation of the LN-308 sublines expressing mutant p53 plasmids in a p53-dependent manner. The p53^R175H-dependent effects are strong over a broad range of concentrations, but p53^R273H-dependent effects are weaker and requires high concentrations of P53R3^[1].
P53R3 induces p53^R248W reactivation is more pronounced proliferation inhibition than observed with p53^R273H. P53R3 does not exhibit cytotoxic effects even at concentrations close to its solubility limit (33 μg/ml)^[1].
P53R3 (33 μg/ml; 18 hours) induces a strong decrease in S phase cells and a G0/G1 cell cycle arrest in LN-308 p53^R175H and LN-308 p53^R273Hcells. But it does not affect cell cycle distribution of LN-308 p53^R248W cells^[1].

592.56

Solid

C₃₂H₃₅Cl₂N₅O₂

922150-12-7

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

DMSO : ≥ 100 mg/mL(168.76 mM)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。