Amifostine thiol (WR-1065) 是细胞保护剂 Amifostine (HY-B0639) 的活性代谢物。Amifostine thiol 是一种具有辐射防护能力的细胞保护剂。Amifostine thiol 通过 JNK 依赖的信号通路激活 p53。
生物活性 | Amifostine thiol (WR-1065) is an active metabolite of the cytoprotector Amifostine (HY-B0639). Amifostine thiol is a cytoprotective agent with radioprotective abilities. Amifostine thiol activates p53 through a JNK-dependent signaling pathway[1][2][3]. |
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体外研究 (In Vitro) | Amifostine thiol can reduce both the direct and delayed detrimental effects of both high and low-LET radiation exposures[1]. Amifostine thiol attenuates both the DNA damage and the G1-phase arrest induced by radiation[1]. Amifostine thiol (4 mM; 30 minutes) protects RKO36 cells from chromosomal damage and death induced by ionizing radiation[1]. Amifostine thiol (4 mM; 30 minutes) protects irradiated RKO36 cells from delayed genomic instability[1]. Amifostine thiol is cytotoxic to RKO36 cells at milimolar concentrations, especially after continuous treatment[1]. Amifostine thiol at 40 μM protects RKO36 cells from delayed genomic instability but not from cell death and immediate chromosomal damage[1]. Amifostine thiol activates JNK resulting in the phosphorylation of p53 at threonine 81[2]. Amifostine thiol affects phosphorylation of topoisomerase IIα leading to changes in enzyme activity and cell cycle progression in CHO AA8 cells[3].
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | 4°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
溶解性数据 | In Vitro: DMSO : 250 mg/mL(1862.34 mM;Need ultrasonic) H2O : 100 mg/mL(744.93 mM;Need ultrasonic) 配制储备液 1 mM | 7.4493 mL | 37.2467 mL | 74.4934 mL | 5 mM | 1.4899 mL | 7.4493 mL | 14.8987 mL | 10 mM | 0.7449 mL | 3.7247 mL | 7.4493 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: PBS Solubility: 16.67 mg/mL (124.18 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在本网站选购。 |