3-Morpholinosydnonimine

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本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

33876-97-0

包装与价格：

包装	价格(元)
100mg	电议
250mg	电议
500mg	电议

产品名称

SIN-1
Linsidomine

产品介绍

3-Morpholinosydnonimine (SIN-1; Linsidomine) 是一种自发的ROS/RNS发生因子和过氧亚硝酸盐供体。3-Morpholinosydnonimine 能够抑制肥大软骨细胞活力，并诱导坏死 (necrosis)。3-Morpholinosydnonimine 诱导p53依赖性凋亡 (apoptosis)，诱导p53积聚和激活MAPK磷酸化。

生物活性

3-Morpholinosydnonimine (SIN-1; Linsidomine) is a spontaneousROS/RNSgenerator and a peroxynitrite donor. 3-Morpholinosydnonimine inhibits hypertrophic chondrocytes activity and inducesnecrosis. 3-Morpholinosydnonimine inducesp53-dependentapoptosis, inducesp53accumulation and activatesMAPKphosphorylation^[1]^[2].

体外研究
(In Vitro)

3-Morpholinosydnonimine (1,3,5 mM; 24 h) 抑制肥厚性软骨细胞活力^[1]。
3-Morpholinosydnonimine (3 mM 和 5 mM; 24 h) 诱导肥大软骨细胞坏死死亡，同时诱导 ATDC5 细胞和 C28/I2 细胞凋亡死亡^[1]。
3-Morpholinosydnonimine (50 μM, 200 μM, 400 μM; 6 h) 在富含神经元的小鼠端脑细胞中诱导 p53 的表达迅速增加，并诱导 p53 依赖的凋亡^[2]。
3-Morpholinosydnonimine (200 μM; 6 h) 通过 p21(ras) 激活诱导 p53 积累^[2]。

Cell Viability Assay^[1]

Cell Line:	Hypertrophic chondrocytes
Concentration:	0 mM, 1 mM, 3 mM, and 5 mM
Incubation Time:	0 h, 4 h, 8 h, 12 h, and 24 h
Result:	Decreased the cellular viability by 30%, 50%, and 80% respectively at 1, 3, and 5 mM.

Western Blot Analysis^[2]

Cell Line:	Mouse primary neural cells
Concentration:	200 μM
Incubation Time:	6 h
Result:	Induced p53 accumulation.

分子量

170.17

Formula

C₆H₁₀N₄O₂

CAS 号

33876-97-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.