Pachybasin is an anthraquinone fungal metabolite. It inhibits the growth of E. coli, S. aureus, B. subtilis, and M. luteus bacteria (MICs = 64, 32, 64, and 64 μg/ml, respectively) and C. albicans, S. cerevisiae, A. niger, A. flavus, and F. oxysporum fungi (MICs = 64, 64, 64, 64, and 16 μg/ml, respectively). It also induces germ tube malformation in B. graminis fungi. Pachybasin induces developmental retardation and notochord distortions and increases mortality in zebrafish embryos when used at concentrations ranging from 1 to 100 μM.