PAA5

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PAA5

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
100mg	电议
250mg	电议
500mg	电议

产品介绍

PAA5 是一种以甲基化物碳为中心的多核 Au(I) 簇。PAA5 能释放 Au(I)，引起促氧化反应，加速铁死亡。PAA5 以时间依赖性方式增加 pH2AX 的表达。PAA5 具有抗癌活性。

生物活性

PAA5 is a methide carbon-centered polynuclear Au(I) cluster. PAA5 can release Au(I) causing Pro-oxidant response and acceleratedferroptosis. PAA5 increases the expression of pH2AX in a time-dependent manner. PAA5 has anticancer activity^[1].

体外研究
(In Vitro)

PAA5 (0-4 μM; 24 h) induces ferroptosis and increase of the ferroptosis marker gene prostaglandin-endoperoxide synthase 2 (PTGS2). PAA5 decreases cell viability in EJ cells with IC₅₀values of 1.0 μM and 2.7 μM for EJ and HUVEC cells, respectively^[1].
PAA5 (1.5 μM; 4 h; EJ and HUVEC cells) releases active Au(I) metabolites within cells and increases GSH and ROS level^[1].
PAA5 (4 μM; 1, 3 and 6 h, EJ cells) causes significant DNA damage as indicated by the increase of histone H2AX phosphorylation (pH2AX)^[1].

Cell Viability Assay^[1]

Cell Line:	HUVEC, EJ cells
Concentration:	0, 0.5, 1, 1.5, 2, and 4 μM
Incubation Time:	24 hours
Result:	Decreased cell viability of EJ cells 57 and 55%, respectively, compare with HUVEC cells.

Western Blot Analysis^[1]

Cell Line:	EJ cells
Concentration:	4 μM
Incubation Time:	1, 3, and 6 hours
Result:	Increased the expression of pH2AX in a time dependent manner.

体内研究
(In Vivo)

PAA5 (1.5 μM, 100 μL; intravesical delivered into the bladder; 5 times every other day for 8 days) shows anti-tumor activity in mouse^[1].

Animal Model:	4-6 weeks, 18 g, female BALB/c nude mice^[1]
Dosage:	1.5 μM, 100 μL
Administration:	Intravesical delivered into the bladder; 5 times every other day for 8 days
Result:	Exhibited a good antitumor effect with the small average tumor volume of 564 ± 180 mm³after 22 days and no significant body weight loss.

分子量

1348.66

Formula

C₁₄H₈Au₅B₂F₈N^2-

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.