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Z-LEHD-FMK
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Z-LEHD-FMK图片
CAS NO:210345-04-3
包装:1mg
市场价:4200元

产品介绍
Z-LEHD-FMK 是一种选择性和不可逆的caspase-9抑制剂,可防止致命的再灌注损伤并减弱细胞凋亡。Z-LEHD-FMK 在大鼠脊髓损伤模型中也表现出神经保护作用。
生物活性

Z-LEHD-FMK is a selective and irreversible inhibitor ofcaspase-9, protects against lethal reperfusion injury and attenuatesapoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma[1][2][3].

IC50& Target[1]

Caspase-9

 

体外研究
(In Vitro)

Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1].
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1].

Apoptosis Analysis[1]

Cell Line:SW480, H460, HCT116 and 293 cells
Concentration:20 μM
Incubation Time:Pretreated for 30 min
Result:Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

Western Blot Analysis[1]

Cell Line:HCT116, SW480 cells
Concentration:20 μM
Incubation Time:2 h
Result:Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.
体内研究
(In Vivo)

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats[2].

Animal Model:Male Wistar albino rats (250-350 g) with SCI[2]
Dosage:0.8 μmol/kg
Administration:I.v. for 1 or 7 days
Result:Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.
分子量

690.72

性状

Solid

Formula

C32H43FN6O10

CAS 号

210345-04-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(144.78 mM;Need ultrasonic and warming)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.4478 mL7.2388 mL14.4776 mL
5 mM0.2896 mL1.4478 mL2.8955 mL
10 mM0.1448 mL0.7239 mL1.4478 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。