您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > hnRNPK-IN-1
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
hnRNPK-IN-1
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
hnRNPK-IN-1图片
CAS NO:2313528-04-8
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
hnRNPK-IN-1 是一种异质核糖核蛋白 K (hnRNPK) 结合配体,用 SPR 和 MST 测量的Kd值分别为 4.6 μM 和 2.6 μM。hnRNPK-IN-1 通过破坏 hnRNPK 和 c-myc 启动子的结合来抑制 c-myc 转录。hnRNPK-IN-1诱导 Hela 细胞凋亡 (apoptosis),并具有很强的抗肿瘤活性。
生物活性

hnRNPK-IN-1 is aheterogeneous nuclear ribonucleoprotein K (hnRNPK)binding ligand withKdvalues of 4.6 μM and 2.6 μM measured with SPR and MST, respectively. hnRNPK-IN-1 inhibitsc-myctranscription by disrupting the binding of hnRNPK andc-mycpromoter. hnRNPK-IN-1 induces Hela cellsapoptosisand has strongly anti-tumor activities[1].

IC50& Target

Kd: 4.6 μM (hnRNPK; SPR assay) and 2.6 μM (hnRNPK; MST assay)[1]

体外研究
(In Vitro)

hnRNPK-IN-1 (Compound 25; 1.25-5 μM; 24 hours) treatment induces Hela cells apoptosis could be due to its repression of cmyc transcription[1].
hnRNPK-IN-1 (1.25-5 μM; 48 hours) treatment down-regulates c-myc gene transcription and expression in Hela cells in a dose-dependent manner[1].
hnRNPK-IN-1 shows a selective anti-proliferative effect on human cancer cell lines (Siha, A549, Hela, U2OS, A375, HuH7 and HEK293 cells) with IC50 values ranged from 1.36 to 3.59 μM[1].
hnRNPK-IN-1 could selectively bind to hnRNP K without significant interaction with c-myc promoter DNA. The binding of hnRNPK-IN-1 to hnRNP K could disrupt the interaction between hnRNP K and c-myc promoter DNA in vitro and in cells[1].

Apoptosis Analysis[1]

Cell Line:Hela cells
Concentration:1.25 μM, 2.5 μM, 5 μM
Incubation Time:24 hours
Result:Induced Hela cells apoptosis and apoptosis-related protein expressions.

RT-PCR[1]

Cell Line:Hela cells
Concentration:1.25 μM, 2.5 μM, 5 μM
Incubation Time:48 hours
Result:Caused a reduction of c-myc mRNA in a dose-dependent manner in Hela cells.

Western Blot Analysis[1]

Cell Line:Hela cells
Concentration:1.25 μM, 2.5 μM, 5 μM
Incubation Time:48 hours
Result:The expression levels of c-MYC also decreased in a dose-dependent manner.
体内研究
(In Vivo)

hnRNPK-IN-1 (Compound 25; 6.7-20 mg/kg; i.p.; once a day; for three weeks) exhibits good tumor growth inhibition in a Hela xenograft tumor model[1].

Animal Model:BALB/c female nude mice (five weeks old) bearing Hela cells[1]
Dosage:6.7 mg/kg, 20 mg/kg
Administration:Intraperitoneal injection; once a day; for three weeks
Result:Inhibited tumor growth in a Hela cervical xenograft.
分子量

419.43

性状

Solid

Formula

C23H21N3O5

CAS 号

2313528-04-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 5 mg/mL(11.92 mM;ultrasonic and warming and heat to 80℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.3842 mL11.9209 mL23.8419 mL
5 mM0.4768 mL2.3842 mL4.7684 mL
10 mM0.2384 mL1.1921 mL2.3842 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。