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L-Theanine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
L-Theanine图片
CAS NO:3081-61-6
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
100mg电议
200mg电议
500mg电议
1 g电议

产品名称
L-茶氨酸
L-Glutamic Acid γ-ethyl amide
Nγ-Ethyl-L-glutamine
产品介绍
L-Theanine (L-Glutamic Acid γ-ethyl amide) 是一种存在绿茶叶片中的非蛋白氨基酸物质,能阻断大脑中谷氨酸与谷氨酸受体结合,具有神经保护、抗癌和抗氧化活性。L-Theanine 能透过血脑屏障,具有口服活性。
生物活性

L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active[1][2][3].

体外研究
(In Vitro)

L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate[3].
L-Theanine (500 μM; 72 h) protects against excessDopamine(HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes[3].
L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis[3].
L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes[4].
L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells[4].
L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells[4].
L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production[5].

Cell Proliferation Assay[4]

Cell Line:A375 and PIG1 cells
Concentration:0.1, 0.5, 1, 2 and 5 mM
Incubation Time:24 h
Result:Dose-dependently decreased the viability of A375, but not PIG1 cells.

Cell Cycle Analysis[4]

Cell Line:A375
Concentration:1, 2 and 5 mM
Incubation Time:24 h
Result:Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase.

Western Blot Analysis[4]

Cell Line:A375
Concentration:1, 2 and 5 mM
Incubation Time:24 h
Result:Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21).
Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2.
Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2.
Dose-dependently increased the p53 expression.

Cell Migration Assay[4]

Cell Line:A375
Concentration:1, 2 and 5 mM
Incubation Time:24 h
Result:Apparently suppressed the migration.

Apoptosis Analysis[4]

Cell Line:A375
Concentration:1, 2 and 5 mM
Incubation Time:24 h
Result:Showed pro-apoptotic effect.

RT-PCR[4]

Cell Line:A375
Concentration:1, 2 and 5 mM
Incubation Time:24 h
Result:Markedly elevated the mRNA levels ofBmal1,Clock,Rora, andRev-erbβ.
体内研究
(In Vivo)

L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice[3].

Animal Model:Healthy male ICR mice weighing 32–34 g[3]
Dosage:4.0 mg/kg
Administration:Oral administration, daily for 14 days
Result:Significantly increased glutathione content in the striatum, but not in the midbrain.
Clinical Trial
分子量

174.20

性状

Solid

Formula

C7H14N2O3

CAS 号

3081-61-6

中文名称

L-茶氨酸;茶氨酸

结构分类
  • Amino acids
来源
  • Plants
  • Theaceae
  • Camellia sinensis(L.) O. Ktze.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 150 mg/mL(861.08 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM5.7405 mL28.7026 mL57.4053 mL
5 mM1.1481 mL5.7405 mL11.4811 mL
10 mM0.5741 mL2.8703 mL5.7405 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。