L-Theanine (L-Glutamic Acid γ-ethyl amide) 是一种存在绿茶叶片中的非蛋白氨基酸物质，能阻断大脑中谷氨酸与谷氨酸受体结合，具有神经保护、抗癌和抗氧化活性。L-Theanine 能透过血脑屏障，具有口服活性。
| 生物活性 | L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active[1][2][3]. |
体外研究
(In Vitro) | L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate[3].
L-Theanine (500 μM; 72 h) protects against excessDopamine(HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes[3].
L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis[3].
L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes[4].
L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells[4].
L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells[4].
L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production[5].
Cell Proliferation Assay[4] | Cell Line: | A375 and PIG1 cells | | Concentration: | 0.1, 0.5, 1, 2 and 5 mM | | Incubation Time: | 24 h | | Result: | Dose-dependently decreased the viability of A375, but not PIG1 cells. |
Cell Cycle Analysis[4] | Cell Line: | A375 | | Concentration: | 1, 2 and 5 mM | | Incubation Time: | 24 h | | Result: | Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase. |
Western Blot Analysis[4] | Cell Line: | A375 | | Concentration: | 1, 2 and 5 mM | | Incubation Time: | 24 h | | Result: | Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21).
Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2.
Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2.
Dose-dependently increased the p53 expression. |
Cell Migration Assay[4] | Cell Line: | A375 | | Concentration: | 1, 2 and 5 mM | | Incubation Time: | 24 h | | Result: | Apparently suppressed the migration. |
Apoptosis Analysis[4] | Cell Line: | A375 | | Concentration: | 1, 2 and 5 mM | | Incubation Time: | 24 h | | Result: | Showed pro-apoptotic effect. |
RT-PCR[4] | Cell Line: | A375 | | Concentration: | 1, 2 and 5 mM | | Incubation Time: | 24 h | | Result: | Markedly elevated the mRNA levels ofBmal1,Clock,Rora, andRev-erbβ. |
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体内研究
(In Vivo) | L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice[3].
| Animal Model: | Healthy male ICR mice weighing 32–34 g[3] | | Dosage: | 4.0 mg/kg | | Administration: | Oral administration, daily for 14 days | | Result: | Significantly increased glutathione content in the striatum, but not in the midbrain. |
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| Clinical Trial | |
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| 来源 | - Plants
- Theaceae
- Camellia sinensis(L.) O. Ktze.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : 150 mg/mL(861.08 mM;Need ultrasonic) 配制储备液 | 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL | | 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL | | 10 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic
*以上所有助溶剂都可在本网站选购。 |
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