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Lucigenin(NSC-151912)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Lucigenin(NSC-151912)图片
CAS NO:2315-97-1
包装与价格:
包装价格(元)
10mM (in 1mL DMSO)电议
100mg电议
500mg电议

产品介绍
Lucigenin (NSC-151912)(L-6868; NSC-151912) 是一种化学发光探针,用于指示细胞中内源性产生的超氧阴离子自由基的存在。
Cas No.2315-97-1
别名光泽精; NSC-151912; L-6868
Canonical SMILESC[N+]1=C2C=CC=CC2=C(C3=C4C=CC=CC4=[N+](C)C5=C3C=CC=C5)C6=C1C=CC=C6.O=N([O-])=O.O=N([O-])=O
分子式C28H22N4O6
分子量510.5
溶解度DMSO : ≥ 25 mg/mL (48.97 mM)
储存条件Store at -20℃, protect from light
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

Lucigenin(L-6868; NSC-151912) is a chemiluminescent probe used to indicate the presence of endogenously generated superoxide anion radicals in cells.Upon single-electron reduction of Lucigenin by a radical species the subsequent form of Lucigenin presents an amplified chemiluminescence which is measurable and can be correlated to the respective amount of reactive radical species present in the system. This approach can be used to probe the natural generation of these species as well as the natural functioning of superoxide dismutase and other enzymes responsible for scavenging radicals. In addition to indicating the presence of radicals in cells, Lucigenin shows interaction with redox-active oxidoreductase enzymes. Lucigenin is shown to mediate the reduction of cytochrome C by xanthine oxidase and to increase the rate of NADPH oxidation. These observations indicate that use of Lucigenin for superoxide analysis is precluded in the presence of high levels of NAD(P)H-oxidoreductases.

[1]. Li Y, et al. Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems. J Biol Chem. 1998 Jan 23;273(4):2015-23. [2]. Rost M, et al. What do we measure with luminol-, lucigenin- and penicillin-amplified chemiluminescence? 1. Investigations with hydrogen peroxide and sodium hypochlorite. J Biolumin Chemilumin. 1998 Nov-Dec;13(6):355-63.