Mensacarcin 是一种高度复杂的聚酮类化合物，在肿瘤细胞系中普遍具有强烈的抑制细胞生长的作用，并可能诱导黑色素瘤细胞的凋亡 (apoptosis)。Mensacarcin 靶向线粒体 (mitochondria)，影响线粒体的能量代谢，激活 caspase 依赖的凋亡途径。Mensacarcin 是一种抗生素，也可作为抗体偶联药物 (ADCs) 的细胞毒性成分。
| 生物活性 | Mensacarcin, a highly complex polyketide, strongly inhibits cell growth universally incancercell lines and potently inducesapoptosisin melanoma cells. Mensacarcin targets tomitochondria, affects energy metabolism in mitochondria, and activates caspase-dependent apoptotic pathways. Mensacarcin, anantibiotic, can be used as a cytotoxic component ofantibody-drug conjugates (ADCs)[1][2]. |
| IC50& Target | Traditional Cytotoxic Agents |
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体外研究
(In Vitro) | Mensacarcin (0-100 μM; 24 hours) exhibits general cytostatic but type-specific cytotoxic effects for melanoma cells[1].
Mensacarcin (2-50 μM; 15 hours) induces rapid apoptotic cell death in melanoma cells[1].
Mensacarcin exhibits potent cytostatic properties (mean of 50% growth inhibition=0.2 μM) in almost all cell lines of the National Cancer Institute (NCI)-60 cell line screen and relatively selective cytotoxicity against melanoma cells. Mensacarcin is a highly oxygenated polyketide that was first isolated from soil-dwellingStreptomycesbacteria. Mensacarcin impairs mitochondrial function in melanoma cells[1].
Cell Viability Assay[1] | Cell Line: | SK-Mel-28 and SK-Mel-5 melanoma cells, HCT-116 colon cancer cells | | Concentration: | 0.01, 0.1, 1, 10, 100 μM | | Incubation Time: | 24 hours | | Result: | Induced concentration- and time-dependent cell death in the two tested melanoma cell lines. HCT-116 colon carcinoma cells were strongly inhibited. |
Western Blot Analysis[1] | Cell Line: | SK-Mel-28, SK-Mel-5 cells | | Concentration: | 2, 10, 50 μM | | Incubation Time: | 15 hours | | Result: | Induced the formation of 89-kDa PARP-1 fragments as well as caspase-3 activation in SK-Mel-28 and SK-Mel-5 beginning between 6 and 15 h after exposure. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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