Cas No. | 207738-08-7 |
别名 | 3,3',5,5'-四甲基苯并啶二盐酸酸水合物 |
Canonical SMILES | CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl.Cl.O.O |
分子式 | C16H20N2•2HCl [2H2O] |
分子量 | 349.3 |
溶解度 | DMSO: 1.7 mg/ml,DMSO:PBS (1:300): 30 µ g/ml |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase. |