| 生物活性 | Berberine sulfate is an alkaloid isolated from the Chinese herbal medicine Huanglian, as anantibiotic. Berberine sulfate inducesreactive oxygen species(ROS) generation and inhibitsDNAtopoisomerase. Berberine sulfate has antineoplastic properties[1]. |
| IC50& Target | ROS[1]
DNA topoisomerase[1] |
体外研究
(In Vitro) | Berberine sulfate (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine sulfate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine sulfate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine sulfate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].
Cell Proliferation Assay[1] | Cell Line: | Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29 | | Concentration: | 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM | | Incubation Time: | 72 hours | | Result: | Inhibited the proliferation of four cell lines. The IC50ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116). |
Cell Proliferation Assay[1] | Cell Line: | Colorectal carcinoma cell lines LoVo | | Concentration: | 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells. |
Cell Cycle Analysis[1] | Cell Line: | LoVo cells | | Concentration: | 0, 10, 20, 40, or 80 μM | | Incubation Time: | 24 hours | | Result: | Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population. |
Western Blot Analysis[1] | Cell Line: | LoVo cells | | Concentration: | 10, 20, 40, or 80 μM | | Incubation Time: | 24 hours | | Result: | Suppressed cyclin B1, cdc2 and cdc25c protein expression. |
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体内研究
(In Vivo) | Berberine sulfate (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].
| Animal Model: | 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1] | | Dosage: | 10, 30, or 50 mg/kg/day | | Administration: | Gastrointestinal gavage; for 10 consecutive days | | Result: | Showed inhibitory rates of 33.1% and 45.3% at doses of 30 and 50 mg/kg/day. |
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| Clinical Trial | |
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| 结构分类 | - Alkaloids
- Isoquinoline Alkaloids
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| 来源 | - Plants
- Ranunculaceae
- Coptis chinensisFranch.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : 50 mg/mL(115.36 mM;Need ultrasonic) DMSO : 0.5 mg/mL(1.15 mM;ultrasonic and warming and heat to 60℃) 配制储备液 | 1 mM | 2.3072 mL | 11.5359 mL | 23.0718 mL | | 5 mM | 0.4614 mL | 2.3072 mL | 4.6144 mL | | 10 mM | 0.2307 mL | 1.1536 mL | 2.3072 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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