Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) 是一种生物碱，常用作抗生素。Berberine chloride hydrate 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。抗肿瘤特性。
| 生物活性 | Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) is an alkaloid that acts as anantibiotic. Berberine chloride hydrate inducesreactive oxygen species(ROS) generation and inhibitsDNAtopoisomerase. Antineoplastic properties[1]. |
| IC50& Target | ROS[1]
DNA topoisomerase[1] |
体外研究
(In Vitro) | Berberine chloride hydrate ( Natural Yellow 18 chloride hydrate; 1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
Berberine chloride hydrate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
LoVo cells are exposure to Berberine chloride hydrate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
Berberine chloride hydrate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].
Cell Proliferation Assay[1] | Cell Line: | Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29 | | Concentration: | 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM | | Incubation Time: | 72 hours | | Result: | Inhibited the proliferation of four cell lines. The IC50ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116). |
Cell Proliferation Assay[1] | Cell Line: | Colorectal carcinoma cell lines LoVo | | Concentration: | 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells. |
Cell Cycle Analysis[1] | Cell Line: | LoVo cells | | Concentration: | 0, 10, 20, 40, or 80 μM | | Incubation Time: | 24 hours | | Result: | Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population. |
Western Blot Analysis[1] | Cell Line: | LoVo cells | | Concentration: | 10, 20, 40, or 80 μM | | Incubation Time: | 24 hours | | Result: | Suppressed cyclin B1, cdc2 and cdc25c protein expression. |
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体内研究
(In Vivo) | Berberine chloride hydrate (Natural Yellow 18 chloride hydrate; 10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine chloride hydrate at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].
| Animal Model: | 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1] | | Dosage: | 10, 30, or 50 mg/kg/day | | Administration: | Gastrointestinal gavage; for 10 consecutive days | | Result: | Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day. |
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| 结构分类 | - Alkaloids
- Isoquinoline Alkaloids
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| 来源 | - Plants
- Ranunculaceae
- Coptis chinensisFranch.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: DMSO : ≥ 3.9 mg/mL(10.00 mM) H2O : 1 mg/mL(2.57 mM;ultrasonic and warming and heat to 60℃) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 2.5652 mL | 12.8261 mL | 25.6522 mL | | 5 mM | 0.5130 mL | 2.5652 mL | 5.1304 mL | | 10 mM | 0.2565 mL | 1.2826 mL | 2.5652 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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