rel-α-Vitamin E (rel-D-α-Tocopherol) is a vitamin with antioxidant properties and also a mixture^[1].

Vitamin E is a lipid-soluble, chain-breaking type of antioxidant present in human blood. Vitamin E works as a free radical scavenger and has the primary function of destroying peroxyl radicals. Thus, it protects long-chain polyunsaturated fatty acids (e.g., cell membranes or low-density lipoprotein cholesterol) from oxidation or destruction. Its association with vitamin C is of great pathophysiological importance, because inhibition of lipid peroxidation by α-tocopherol occurs through its conversion into an oxidized α-tocopheroxyl radical, which in turn is regenerated to α-tocopherol through reduction by redox-active reagents^[1].
α-Tocopherol can inhibit the recruitment of lymphocytes and eosinophils^[1].

In order to longitudinally relate vitamin E status, evaluated by means of α- and γ-tocopherol measured in erythrocytes membranes, to inflammatory state and oxidative stress by means of total antioxidant capacity of plasma and lipid peroxidation biomarkers, and to assess its association to hard long-term outcomes (e.g., overall and cause-specific graft and recipient loss), and to be able to likewise account for liver, lung, and heart transplant recipients^[1].

430.71

Oil

C₂₉H₅₀O₂

2074-53-5

• Phenols
• Monophenols

• Others

• Plants
• other families

Room temperature in continental US; may vary elsewhere.

Ethanol : 50 mg/mL(116.09 mM;Need ultrasonic)

DMSO : 16.11 mg/mL(37.40 mM;Need ultrasonic)

H₂O :< 0.1 mg/mL(insoluble)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 1.61 mg/mL (3.74 mM); Clear solution

  此方案可获得 ≥ 1.61 mg/mL (3.74 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 16.1 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 1.61 mg/mL (3.74 mM); Clear solution

  此方案可获得 ≥ 1.61 mg/mL (3.74 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 16.1 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。