Methicillin sodium salt (Meticillin sodium) 是一种 β-内酰胺，半合成青霉素类抗生素。Methicillin sodium salt 抑制青霉素结合蛋白参与肽聚糖的合成。Methicillin sodium salt 能够抑制金黄色葡萄球菌，MIC 值为 2.1 μg/mL。Methicillin sodium salt 可用于炎症研究。
| 生物活性 | Methicillin sodium salt (Meticillin sodium) is aβ-lactam, semi-syntheticantibioticrelated to penicillinantibiotic. Methicillin sodium salt inhibits penicillin-binding proteins involved in the synthesis of peptidoglycan. Methicillin sodium salt inhibitsS. aureuswith a MIC value of 2.1 μg/mL. Methicillin sodium salt can be used for the research of inflammation[1][2]. |
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体外研究
(In Vitro) | Methicillin sodium salt (100 μg/mL; 18 h) killsS. aureusafter incubation intracellular for 18 hours[1].
Cell Viability Assay[1] | Cell Line: | Leukocytes | | Concentration: | 100 μg/mL | | Incubation Time: | 18 hours | | Result: | Effectively eliminatedS. aureuswith minimal bactericidal concentration of 3.13 μg/mL, and exhibited better effects under aerobical condition. |
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体内研究
(In Vivo) | Methicillin sodium salt (42.5 and 85 mg/kg; i.m. four times daily; for 21 days or till spontaneous death) against the enterococcus in leukocytes[1].Methicillin sodium salt (400 mg/kg; i.p. once) survives infected mice better than compared group[2].Methicillin sodium salt (400 mg/kg; i.h. once) prevents infected mice from death[2].
| Animal Model: | White New Zealand rabbits, weighing 2.0 to 3.0 kg[1] | | Dosage: | 42.5 and 85 mg/kg | | Administration: | Intramuscular injection; 42.5 and 85 mg/kg four times daily; for 21 days or till spontaneous death | | Result: | Inhibited enterococcus at 24 hours with a half-live of 1.1 h, but showed no statistical
significance to rabbits. |
| Animal Model: | Mice with 5-7 X 108S. aureus[2] | | Dosage: | 400 mg/kg | | Administration: | Intraperitoneal injection; 400 mg/kg; once | | Result: | Showed a survival rate of 30.5%, higher than compared group with a survival rate of 6.9%. |
| Animal Model: | Mice with 5-7 X 108S. aureus[2] | | Dosage: | 400 mg/kg | | Administration: | Subcutaneous injection; 400 mg/kg; once | | Result: | Suppressed large abscesses developed in mice, and also prevented mice from death. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: DMSO : 125 mg/mL(310.64 mM;Need ultrasonic) H2O : 100 mg/mL(248.51 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL | | 5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL | | 10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 100 mg/mL (248.51 mM); Clear solution; Need ultrasonic 2. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.17 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 3. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.17 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 4. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (5.17 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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