Natamycin (Pimaricin) is amacrolideantibioticagent produced by severalStreptomycesstrains. Natamycin inhibits the growth offungivia inhibition of amino acid and glucose transport across the plasma membrane. Natamycin is a food preservative, an antifungal agent in agriculture, and is widely used forfungalkeratitis research^[1][2].

Natamycin, an important macrolide antibiotic, produced by several Streptomyces species, includingS. natalensis,S. gilvosporeus,S. lydicusandS. chattanoogensis, can inhibit the growth of fungi via immediate inhibition of amino acid and glucose transport across the plasma membrane. Natamycin is widely used in the food industry and in pharmacotherapy for topical treatment. Unlike other polyene antibiotics, the mode of action of Natamycin is not based on the ergosterol-dependent permeabilization of the plasma membrane. The immediate cessation of growth of yeasts by Natamycin treatment indicates that there might be an instantaneous effect of Natamycin at the level of the plasma membrane^[1][2].

665.73

Solid

C₃₃H₄₇NO₁₃

7681-93-8

纳他霉素；游霉素；匹马菌素；那他霉素

• Antibiotics
• Macrolide Antibiotics

streptomycetes

Room temperature in continental US; may vary elsewhere.

DMSO : 16.67 mg/mL(25.04 mM;ultrasonic and adjust pH to 6 with HCl)

H₂O : 1 mg/mL(1.50 mM;ultrasonic and warming and heat to 80℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 1.67 mg/mL (2.51 mM); Clear solution

  此方案可获得 ≥ 1.67 mg/mL (2.51 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

  Solubility: ≥ 1.67 mg/mL (2.51 mM); Clear solution

  此方案可获得 ≥ 1.67 mg/mL (2.51 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明