Framycetin sulfate (Neomycin B sulfate), anaminoglycosideantibiotic, is a potentRNase P cleavage activityinhibitor with aK_iof 35 μM. Framycetin sulfate competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin sulfate inhibitshammerhead ribozymewith aK_iof 13.5 μM. Framycetin sulfate, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenicE. coliinfections^[1][2].

The inhibition of RNase P RNA cleavage by Framycetin sulfate (Neomycin Bsulfate; Fradiomycin Bsulfate) is sensitive to pH and an increase in pH suppresses the inhibition in other systems^[1].
Framycetin sulfate targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin sulfate selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line^[2].
Framycetin sulfate binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function^[3].
Framycetin sulfate induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region^[4].

908.88

Solid

C₂₃H₅₂N₆O₂₅S₃

4146-30-9

新霉素B硫酸盐；弗氏菌丝素硫酸盐；弗拉霉素硫酸盐

Room temperature in continental US; may vary elsewhere.

H₂O : 250 mg/mL(275.06 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 50 mg/mL (55.01 mM); Clear solution; Need ultrasonic