Kanamycin (Kanamycin A) 是一种口服有效的抗菌剂 (革兰氏阴性/阳性细菌)，可通过与 70 S 核糖体亚单位结合，抑制转位并导致编码错误。Kanamycin 对M. tuberculosis(敏感或耐药) 以及K. pneumonia均具有较好的抑制活性，可用于肺结核和肺炎的研究。
| 生物活性 | Kanamycin (Kanamycin A) is an orally active antibacterial (gram-negative/positive bacteria) agent, inhibits translocation and causes misencoding by binding to the 70 S ribosomal subunit. Kanamycin shows good inhibitory activity to bothM. tuberculosis(sensitive and drug-resistant ) andK. pneumonia, which can be used in studies of tuberculosis and pneumonia[1][2][3][4]. |
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体外研究
(In Vitro) | Kanamycin (0.1-100 μg/mL; 2 weeks) exhibits good antibacterial activity (MIC=1-5 μg/mL) to various strains ofmycobacteriain vitro[1].
Cell Viability Assay[1] | Cell Line: | mycobacteriaH37Rv, H2, H37RvR-PAS, Ravenel, Kirchbergand and BCG. | | Concentration: | 0.1-100 μg/mL | | Incubation Time: | 2 weeks | | Result: | Showed good antibacterial activity to various strains ofmycobacteria(H37Rv, H2, H37RvR-PAS, Ravenel, and BCG) with MICs were 1 μg/mL and 5 μg/mL for strain of Kirchbergand. |
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体内研究
(In Vivo) | Kanamycin (2, 4 mg/kg; s.c.; once daily, 6 times a week for 3 weeks) inhibits growth ofbovine tubercle bacilliin lung and spleen of mice[1].
Kanamycin (1.25, 5 mg/kg; s.c.; single (at 3 h after infection)) inhibits the multiplication ofK. pneumoniaDT-S in lung, trachea, and blood of mice and in proportion to the dose administration, and also increases the survival rate of mice[2].
| Animal Model: | Inbred strain normal mice (14-16 g;bovine tubercle bacilli(Ravenel strain) infected model)[1]. | | Dosage: | 2, 4 mg/kg | | Administration: | Subcutaneous injection; once daily, 6 times a week for 3 weeks. | | Result: | Exerted a marked effect to inhibit the multiplication of thetuberculosisin vivo, especially in the lung of mice. |
| Animal Model: | Slc:ICR male mice (4-week-old; 18-24 g;K. pneumoniaDT-S infection model (by the aerosol method))[2]. | | Dosage: | 1.25, 5 mg/kg | | Administration: | Subcutaneous administration; single (at 3 h after infection). | | Result: | Suppressed the growth ofK. pneumoniaDT-S in lung, trachea, and blood in proportion to the dose administration.
Resulted in 90% survival at 6 days after infection (negative control group: all died within 4 days), and cleared theK. pneumoniaDT-S from lung, trachea, and blood of mice within 48 h (when dosage at 5 mg/kg). |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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