Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λ_ex=488 nm, λ_em=525 nm)^[1].

In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA^[1].

346.38

Solid

C₂₁H₁₈N₂O₃

109244-58-8

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

DMSO : 100 mg/mL(288.70 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

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  Description: Dihydrorhodamine 123 can be used as a fluorescence probe for detecting ROS and ER stress with green fluorescence.
  Method: For cell labeling.
  1. Incubate cells with Dihydrorhodamine 123 (10 μM; 20 min).
  2. Use a confocal laser scanning microscopy for image (Olympus, Japan).
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  Description: Dihydrorhodamine 123 can be used as a fluorescence probe for detecting ion in cells with green fluorescence.
• 
  Description: Dihydrorhodamine 123 can be used as a fluorescence probe for detecting ROS with green fluorescence.
  Method: For cell labeling.
  1. Incubate cells with Dihydrorhodamine 123 (10 μM; 30 min).
  2. Use a laser confocal microscope for image (Delta Vision TM Elite, GE).