4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkalinephosphatasefluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Serum acid phosphatase assay^[1]:
1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.
2. Add the following agents (with the final concentration) 20 mMSodium Tartrate(HY-128476), 15 mMSodium Fluoride(HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM1, 10-Phenanthroline(HY-W004544), 6 mM8-Quinolinol(HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mMEDTA(HY-Y0682) and 6 mMEGTA(HY-D0861).
3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.
4. Mix samples thoroughly and determine fluorescence by a fluorometer.

256.15

Solid

C₁₀H₉O₆P

3368-04-5

4-甲基伞形酮磷酸酯

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

H₂O : 20.83 mg/mL(81.32 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 10 mg/mL (39.04 mM); Clear solution; Need ultrasonic