5-TAMRA-SE is an amine-reactive fluorescent agent, its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability.

527.52

Solid

C₂₉H₂₅N₃O₇

150810-68-7

5-羧基四甲基罗丹明琥珀酰亚胺酯

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

DMF : 10 mg/mL(18.96 mM;Need ultrasonic)

DMSO : 5 mg/mL(9.48 mM;Need ultrasonic and warming)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMF    90% (20%SBE-β-CDin saline)

  Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution

• 
  Description: 5-TAMRA-SE (5-TAMRA-NHS ester) is a fluorescent agent with red fluorescence, it can be labeled to gene vectors/DNA for observing cellular uptake.
  Method: For cell labeling.
  1. The gene vectors/DNA NPs are labeled with 5-TAMRA-SE dye.
  2. Cells are seeded into the glass plates. After 20 h culture, the cell membrane is labeled with DIO dye and then treated with the same amount of the vectors/DNA NPs in 1 mL fresh RPMI 1640 medium without FBS.
  3. The living cells are immediately observed by TIRFM (Olympus, Japan). The fluorescence images are captured every 5 s, and the nanoparticles are analyzed by single-particle tracking (SPT) technique programmed by MATLAB software.