Fast-TRFS is a selective and superfast fluorogenic probe ofthioredoxin reductase (TrxR). Fast-TRFS can be used for imagingTrxRactivity in live cells^[1].

Fast-TRFS reaches the maximal fluorescence signal within 1 min incubated with TCEP, and within 5 min incubated with the TrxR enzyme^[1].
Fast-TRFS (10 μM; 2-60 min) appears the blue fluorescence signal within 2 min in HeLa cells^[1].

376.37

Solid

C₁₄H₁₁F₃N₂O₃S₂

Room temperature in continental US; may vary elsewhere.

DMSO : 100 mg/mL(265.70 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: 2.5 mg/mL (6.64 mM); Suspended solution; Need ultrasonic

  此方案可获得 2.5 mg/mL (6.64 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液

• 
  Description: Fast-TRFS is a disulfide containing fluorescent probe which yields fluorescence only when its disulfide bond is reduced to dithiols. Fast-TRFS can be used in a fast detection of mammalian TrxR activity.
  Method: For determine location of Fast-TRFS inE.coli.
  1. To detect Fast-TRFS, mid-log phaseE.colibacteria are treated with the probe for 5 min.
  2. Then, bacterial cells, at concentrations of 10⁶colony forming units (CFU)/mL, are transferred to slides for observation under the confocal microscope (Nikon, Japan).
• 
  Description: Fast-TRFS is a disulfide containing fluorescent probe which yields fluorescence only when its disulfide bond is reduced to dithiols. Fast-TRFS can be used in a fast detection of mammalian TrxR activity.
  Method: For Fast-TRFS assay.
  1. 10 μM of Fast-TRFS is added to both untreated and TrxR inhibitors treated bacteria.
  2. The TRFS fluorescence intensity is measured using a multimode reader (M200 Tecan, Austria). Images of cells are taken under inverted fluorescent microscope (Nikon, japan).