Calcein Blue, a membrane-impermeantfluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator^[1][2][3].

The fluorescence of Calcein Blue (CB) is quenched by the Fe²⁺ion in the Calcein Blue-Fe²⁺complex. When dopamine is added to a solution of the Calcein Blue-Fe²⁺complex, a dopamine-Fe²⁺complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe²⁺complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe²⁺complex shows a high selectivity for dopamine among the catecholamines and related compounds examined^[1].

321.28

Solid

C₁₅H₁₅NO₇

54375-47-2

钙黄绿素蓝

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DMSO : 6 mg/mL(18.68 mM;Need ultrasonic and warming)

H₂O : 1 mg/mL(3.11 mM;ultrasonic and warming and heat to 80℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。