ODN 2007 为 B 类 CpG ODN (寡脱氧核苷酸), 是一种 Toll 样受体 (TLR) 配体。ODN 2007 可作为免疫调节剂、疫苗佐剂，增强哺乳动物、鱼类和人类的免疫反应。ODN 2007 序列：5'-TCGTCGTTGTCGTTTTGTCGTT-3'。
| 生物活性 | ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is aToll-like receptor(TLR) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3'[1][2][3]. |
体外研究
(In Vitro) | ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression[1].
ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO[3].
RT-PCR[1] | Cell Line: | MQ-NCSU cells (a chicken macrophage cell line) | | Concentration: | 1 μg/mL, 5 μg/mL | | Incubation Time: | 3 h, 12 h, 18 h | | Result: | Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose.
Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point. |
Western Blot Analysis[3] | Cell Line: | HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line | | Concentration: | 10 μg/mL | | Incubation Time: | 0.5 h, 1 h, 3 h, 6 h, 12 h | | Result: | Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels.
Stimulated an increase in the level of NO. |
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体内研究
(In Vivo) | ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected withVibrio traumaticusFJ03-X2[2].
| Animal Model: | Zebrafish infected withVibrio traumaticusFJ03-X2[2] | | Dosage: | 1 μg | | Administration: | Intraperitoneal injection; once | | Result: | Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%.
Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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