ODN 1668 是 B 类 CpG ODN (寡脱氧核苷酸)，是一种TLR-9激动剂。 ODN 1668 是一种免疫刺激序列，可作为疫苗佐剂。 序列：5’-tccatgacgttcctgatgct-3’。
| 生物活性 | ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is aTLR-9agonist. ODN 1668 is an immunostimulatory sequence and can be used as vaccine adjuvant. Sequence: 5'-tccatgacgttcctgatgct-3’[1][2]. |
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体外研究
(In Vitro) | ODN 1668 induces TNF-α secretion and promotes polyclonal B cell activation[1].
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体内研究
(In Vivo) | ODN 1668 (10 nmol) stimulates responses to protein antigen[1].
ODN 1668 (1 or 5 mg/kg; i.p. or s.c.; once) causes moderate fever and anorexia in rats[2].
| Animal Model: | C57BW6 mice[1] | | Dosage: | 10 nmol | | Administration: | 300 μg OVA (ovalbumin) in PBS or liposomes containing OVA were injected with or without 10nmol ODN in the hind footpads of C57BW6 mice. A boost of the same
inoculum was given at 2 weeks, and 1 week later blood was taken for serum antibody titering. | | Result: | Strongly potentiated the antibody response and induced class switching toward IgG2a and IgG2b. Showed B cell blast formation and a more than twofold increase in B7.2 (CD86) and IL-2 receptor-α (CD25) surface expression. |
| Animal Model: | Male Wistar rats with body weights in the range of 175–200 g[2] | | Dosage: | 1 mg/kg or 5 mg/kg | | Administration: | Intraperitoneal and subcutaneous injection, once | | Result: | Caused moderate fever and anorexia. Induced a significant increase of IL-6. Increased expression of inflammatory genes and activated inflammatory transcription factors. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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