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MAFP
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
MAFP图片
CAS NO:188404-10-6
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
Methyl Arachidonyl Fluorophosphonate
产品介绍
MAFP (Methyl Arachidonyl Fluorophosphonate) 是选择性,针对活性位点,不可逆的cPLA2iPLA2抑制剂。 MAFP也是一种有效的不可逆的anandamide amidase抑制剂。
生物活性

MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor ofcPLA2andiPLA2. MAFP is also a potent irreversible inhibitor ofanandamide amidase.

IC50& Target

cPLA2, iPLA2[1], Anandamide amidase[2]

体外研究
(In Vitro)

MAFP inhibits iPLA2, in a concentration-dependent manner with an IC50of 5 μM after a 5 min preincubation at 40℃ in P388D1 cells. cPLA, is a phospholipid hydrolase using the hydroxyl of serine-228 residue as its catalytic nucleophile[1]. MAFP is also an inhibitor of anandamide amidase and as a ligand for the CB1 cannabinoid receptor. MAFP demonstrates selectivity towards anandamide amidase for which it is approximately 3000 and 30000-fold more potent than it is towards chymotrypsin and trypsin, respectively. MAFP displaces [3H]CP-55940 binding to the CB1 cannabinoid receptor with an IC50of 20 nM vs 40 nM for anandamide[2].

分子量

370.48

性状

Liquid

Formula

C21H36FO2P

CAS 号

188404-10-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Pure form-20°C3 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL(269.92 mM)

Methyl Acetate : ≥ 10 mg/mL(26.99 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.6992 mL13.4960 mL26.9920 mL
5 mM0.5398 mL2.6992 mL5.3984 mL
10 mM0.2699 mL1.3496 mL2.6992 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。