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Compound 48/80 trihydrochloride
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Compound 48/80 trihydrochloride图片
CAS NO:848035-21-2
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
50mg电议
100mg电议
200mg电议
500mg电议

产品名称
C48/80 trihydrochloride
产品介绍
Compound 48/80 trihydrochloride (C48/80 trihydrochloride) 是 N-甲基对甲氧基苯乙胺与甲醛缩合产物的混合物。Compound 48/80 trihydrochloride 也是一种组胺(histamine)释放剂和肥大细胞(mast cell)脱颗粒剂。Compound 48/80 trihydrochloride 抑制人血小板磷脂酰肌醇特异性磷脂酶 C (phosphatidylinositol-specific phospholipase C) 活性。
生物活性

Compound 48/80 trihydrochloride (C48/80 trihydrochloride) is a mixture of condensation products of N-methyl-p-methoxyphenethylamine with formaldehyde. Compound 48/80 trihydrochloride is also ahistaminereleaser and amast celldegranulator. Compound 48/80 inhibitsphosphatidylinositol-specificphospholipaseCactivity from human platelets[1][2][3].

体外研究
(In Vitro)

Compound 48/80 trihydrochloride (C48/80 trihydrochloride) inhibits both cytosolic and particulate phosphatidylinositol-specific phospholipase C activities with a similar efficiency; IC50values are 2.1 μg/ml (supernatant) and 5.0 μg /ml (particulate fraction). The aggregation of human platelets induced by ADP and PAF-acether is inhibited by Compound 48/80[1].

体内研究
(In Vivo)

Compound 48/80 trihydrochloride (0.75 mg/kg; i.p.; Killed after 0.5, 3 or 6 h) increases serum serotonin, histamine and corticosterone levels at 0.5 h, but their increases were reduced thereafter[3].
Compound 48/80 trihydrochloride causes oxidative stress in rat adrenal gland through mast cell degranulation[3].

Animal Model:7-week-old Wistar male rats (fasted for 24 hours)[3]
Dosage:0.75 mg/kg
Administration:Intraperitoneal injection; Killed after 0.5, 3 or 6 h
Result:Serum histamine and serotonin concentrations significantly higher than those in untreated control rats at 0.5 h after treatment. The increased serum histamine and serotonin concentrations in rats decreased time-dependently thereafter.
分子量

629.10

性状

Solid

Formula

C32H48Cl3N3O3

CAS 号

848035-21-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 25 mg/mL(39.74 mM;Need ultrasonic)

DMSO : 10 mg/mL(15.90 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.5896 mL7.9479 mL15.8957 mL
5 mM0.3179 mL1.5896 mL3.1791 mL
10 mM0.1590 mL0.7948 mL1.5896 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (3.97 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.97 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (3.97 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.97 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在本网站选购。