您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 产品库 > Tat-NR2B9c
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Tat-NR2B9c
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Tat-NR2B9c图片
CAS NO:500992-11-0
包装:5mg, 10mg
包装与价格:
包装价格(元)
5mg电议
10mg电议

产品介绍

生物活性

Tat-NR2B9c is a 20-aa peptide, it acts as a postsynaptic density-95 (PSD-95) inhibitor with EC50 of 6.7 nM for PSD-95d2. Tat-NR2B9c also reduces NMDA-induced p38 activation, and possesses neuroprotective efficacy. Tat-NR2B9c inhibits NMDAR2A, NMDAR2B, and NMDAR2C binding to PSD-95, with IC50s of 0.5 μM, ~8 μM, and 0.75 μM, respectively. Tat-NR2B9c also blocks the interaction between PSD-95 and nNOS with an IC50 of ~0.2 μM. In male C57BL/6 mice, Tat-NR2B9c (10 nM/g; i.v.) reduces infarction volume , but has no effect at 3 nM/g.


化学数据

分子量2518.88
分子式C105H188N42O30
CAS号500992-11-0
纯度>98%
溶解性(25°C)Water 30 mg/mL
储存和运输条件-20°C, sealed
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系Postnatal mono-cultured WT and YAC128 striatal neurons
方法Postnatal mono-cultured WT and YAC128 striatal neurons are pretreated for 1 h with 200 nM Tat-NR2B9c, and/or SB-239063 (p38 inhibitor), and/or SP-600125 (JNK inhibitor), then incubated with or without 500 μM NMDA for 10 min. After NMDA treatment, striatal neurons are washed once with warm plating medium (PM) and then incubated in conditioned PM (without Tat peptides or p38, JNK inhibitors) for 24 h. Then cells are washed with PBS once and fixed with 4% paraformaldehyde (PFA) for 30 min.
浓度200 nM
处理时间1 h

动物实验
动物模型Mice
配制-
剂量0.0, 3.0, 10.0 nMole/g
给药处理intravenously via the tail vein using a pump in a volume of 1 μL/g

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM0.397 mL1.985 mL3.97 mL
5 mM0.0794 mL0.397 mL0.794 mL
10 mM0.0397 mL0.1985 mL0.397 mL